Thermo Scientific Vanquish Flex Quaternary UHPLC-MS System with ISQ EC Mass Spectrometer & VWD Detector

Quaternary UHPLC System: Features a four-solvent quaternary pump (VF-P20-A) with a 350 µL static mixer enabling low-pulsation, highly accurate solvent delivery (±0.1% flow accuracy) over a broad flow range of 0.001–8 mL/min with pressure capability up to 103 MPa. Built-in solvent degassing and low-pressure gradient formation support reproducible gradient elution critical for bioprocessing and LC-MS method development.

Variable Wavelength Detector (VWD): The Vanquish Variable Wavelength Detector F (VF-D40-A) employs a tunable Czerny-Turner monochromator and an internal reference beam for UV/VIS absorbance measurements from 190–900 nm. It supports up to four simultaneous signal channels with spectral bandwidth ~6 nm, wavelength accuracy ±1 nm, and low noise (<±2.5 µAU single-channel at 254 nm). This detector is essential for fluorescence analysis and quality control in synthetic biology workflows.

ISQ EC Mass Spectrometer: Equipped with a heated electrospray ionization (HESI) source, delivering sensitive mass detection from m/z 10 to 1250 at unit resolution. It offers fast scan rates up to 20,000 Da/s, multiple data acquisition modes including Full Scan and SIM with high sensitivity (e.g., 10 pg Reserpine detection at S/N ≥400:1), and rapid polarity switching (25 ms). The DynaMax XR detector provides a ≥10⁷ dynamic range, supporting molecular diagnostics and biopharmaceutical production pipelines.

Split Sampler Autosampler: The Vanquish Split Sampler FT (VF-A10-A) features split loop injection with volumes from 0.01 to 25 µL, exceptional injection precision (<0.25% RSD for 1 µL injections), and temperature-controlled sample handling from 4–40 °C with ±1 °C stability. This autosampler ensures reproducible injections crucial for high-throughput screening and gene editing research.

Column Compartment with Active Preheater: The Vanquish Column Compartment H (VH-C10-A) maintains isothermal conditions for chromatography columns with still and forced air temperature regulation from 5 to 120 °C (±0.05 K stability). The active preheater supports temperatures up to 120 °C with rapid heating and cooling capabilities, enabling robust separations critical in biomanufacturing and small molecule impurity analysis.

Additional Components: Includes a high-quality Edwards external vacuum pump for the MS system, standard flow cells (11 µL, 10 mm path length, stainless steel), Chromeleon software (v7.2.10 ES) on a Windows 10 PC with a USB license dongle, and comprehensive interconnect cables.