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Thermo Scientific NanoDrop 2000 Microvolume UV-Vis Spectrophotometer for Nucleic Acid and Protein Quantification
Product Description
The Thermo Scientific NanoDrop 2000 is a highly precise microvolume UV-Vis spectrophotometer designed for rapid concentration and purity assessments of nucleic acids and proteins. Utilizing a pedestal sample platform, it requires minimal volumes of only 0.5 to 2.0 µL, supporting efficient workflows in molecular biology, biotechnology, and clinical diagnostics.
Key technical specifications include:
- Wavelength range: 190–840 nm, ideal for absorbance measurements relevant to DNA, RNA, and protein quantification
- Detector: 2048-element linear silicon CCD array with spectral resolution ≤ 1.8 nm ensuring accurate spectral data
- Light source: Xenon flash lamp for rapid, stable illumination
- Measurement time: less than 5 seconds per sample, facilitating high-throughput sample verification in gene editing and bioprocessing workflows
- Detection limits: down to 2 ng/µL for dsDNA and 0.10 mg/mL for BSA protein concentration
- Absorbance accuracy: 3% at 0.74 absorbance (350 nm), supporting reliable purity ratio calculations critical in synthetic biology and molecular cloning
- Connectivity: USB interface for seamless data transfer and integration with laboratory information management systems (LIMS)
The NanoDrop 2000's compact benchtop design and single-sample capability make it an essential instrument widely adopted in biomanufacturing, molecular diagnostics, and fluorescence analysis labs. Its microvolume analysis avoids sample dilution or cuvette use, reducing reagent consumption and streamlining nucleic acid extraction, PCR setup, and sequencing preparation processes.
Overall, this spectrophotometer supports key biotechnological applications by delivering fast, reproducible measurements of sample concentration and purity, making it critical in research areas such as synthetic biology, biomedical engineering, and clinical genomics.
Item Stats
Product details
| Absorbance Accuracy | 3% (at 0.74 Abs at 350 nm) |
| Absorbance Range | Pedestal: 0 to 300 A Cuvette: 0 to 1.5 A |
| Detection Limits | Pedestal: 2 ng/μL (dsDNA), 0.10 mg/mL (BSA) Cuvette: 0.4 ng/μL (dsDNA), 0.01 mg/mL (BSA) |
| Detection Range | 2–15,000 ng/µL (dsDNA), 0.10–400 mg/mL (BSA) |
| Detector Type | 2048-element linear silicon CCD array |
| Spectral Resolution | ≤ 1.8 nm (FWHM at Hg 253.7) |
| Wavelength Accuracy | ±1 nm |
| Wavelength Range | 190–840 nm |
| No. of Samples | 1 |
| Sample Volume (Metric) | 0.5 to 2.0 μL |
| Measurement Time | < 5 sec. |
| Lamp | Xenon Flash |
| Connections | USB |
| Power | 12 VDC, 5 W |
| Dimensions | 9 x 6 x 8 in |
| Weight | 11 lbs |
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