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Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader for Absorbance and Fluorescence Assays
Product Description
The Molecular Devices SpectraMax M5 is a high-performance, multi-mode microplate reader engineered for diverse biochemical and biotechnological assays. This versatile system supports absorbance, fluorescence intensity, luminescence, time-resolved fluorescence, and fluorescence polarization detection across plate formats from 6 to 384 wells, including cuvette measurements.
Key technical capabilities include:
- Dual monochromators with 1 nm wavelength resolution covering a wide spectral range (200–1000 nm) eliminating filter swaps to streamline method development.
- Sensitive detection through two photomultiplier tubes (PMTs) and a xenon flash lamp light source (1 joule/flash).
- Temperature control from 2 °C above ambient to 60 °C with uniformity under 1 °C at 37 °C, ideal for kinetic and endpoint assays.
- Integrated shaking module adjustable from 0 to 999 seconds to support homogeneous assay conditions.
- Fast read times: approximately 18 seconds for 96-well absorbance reads and less than a minute for 384-well plates, enabling enhanced throughput.
- Photometric accuracy of < ±0.006 OD over 0–2 OD range for microplates, high precision fluorescence with sensitivity down to ≤ 1 pM fluorescein (96 wells) and luminescence detection sensitivity ≤ 43 pM ATP.
- Software integration using SoftMax Pro for data acquisition, curve fitting, regression models, and compliance with audit trails, supporting molecular diagnostics and biotechnology workflows.
- Compatibility with robotic automation and barcode workflows, facilitating seamless integration into high-throughput laboratory settings.
This multi-mode plate reader is widely adopted in biotechnology, molecular biology, and clinical diagnostics, providing critical support for bioprocessing, gene editing validation, synthetic biology assays, and biopharmaceutical production pipelines. Its multi-detection capability eliminates the need for multiple instruments, optimizing lab space and workflow reliability.
Dimensions of 22 x 16 x 9 inches and a weight of 36 lbs make it benchtop suitable, with power requirements compliant with standard laboratory infrastructure (<420 watts, 100-240 VAC).
Overall, the SpectraMax M5 is a robust, flexible microplate reader essential for researchers requiring precise quantitative measurements across multiple fluorescence and absorbance modalities, critical in live-cell imaging assays, fluorescence analysis, and molecular cloning workflows.
Item Stats
Product details
| Read modes | Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization |
| Robotic-compatible | Yes |
| Plate formats | 6, 12, 24, 48, 96, 384 wells |
| Light source | Xenon Flash Lamp (1 joule/flash) |
| Detectors | 2 photomultiplier tubes (PMT) |
| Shaker time | 0 to 999 seconds |
| Temp. control | 2°C above ambient to 60°C |
| Temp. uniformity | < 1°C at 37°C set point |
| Temp. accuracy | ±1°C at 37°C set point |
| Readings | Endpoint, Kinetic Spectral Scanning |
| Standard Read Times (minutes: seconds)* 96 wells 384 wells | Absorbance 0:18 0:49 Fluorescence Intensity 0:17 0:48 Luminescence 2:00 7:00 |
| Reading Capabilities | Cuvette or microplate |
| Wavelength range | 200–1000 nm |
| Wavelength selection | Monochromator, tunable in 1.0 nm increments |
| Wavelength bandwidth | ≤ 4.0 nm |
| Wavelength accuracy | ±2.0 nm |
| Wavelength repeatability | ±0.2 nm |
| Photometric range | 0–4.0 OD |
| Photometric resolution | 0.001 OD |
| Photometric accuracy | (microplate) < ±0.006 OD ±1.0%, 0–2 OD (cuvette) < ±0.005 OD ±1.0%, 0–2 OD |
| Photometric precision | < ±0.003 OD ±1.0%, 0–2 OD |
| Stray light | < 0.05% @ 230 nm |
| Fluorescence Intensity Performance | Top or bottom |
| Wavelength range | 250–850 nm |
| Wavelength selection | Monochromators, tunable in 1.0 nm increments |
| Bandwidth (EX, EM) | 9 nm, 15 nm |
| Optimized sensitivity | ≤ 1 pM fluorescein in 96 wells, ≤ 1.5pM in 384 wells |
| Guaranteed sensitivity | < 5 pM fluorescein in 96 wells or cuvette, < 20 pM in 384 wells |
| Luminescence Performance | Top or Bottom |
| Wavelength selection | Choice of simultaneous detection of all wavelengths or selection via monochromator, tunable in 1.0 nm increments |
| Wavelength range | 250–850 nm |
| Optimized sensitivity | ≤ 43pM ATP in 96 wells |
| Guaranteed sensitivity | ≤ 75pM ATP in 96 wells |
| Dynamic range | > 6 decades |
| Cross-talk | < 0.3% in white 96- and 384-well Microplates |
| Time Resolved Fluorecesnce Performance | Top or Bottom of a Microplate |
| Wavelength selection Monochromators, | tunable in 1.0 nm increments |
| Bandwidth (EX, EM) | 9 nm, 15 nm |
| Precision data collection | 1–100 flashes, delay of 0–600 µsec. before read, integration time selectable between 50–1500 µsec. |
| Optimized sensitivity | ≤ 10 fM europium in 96 |
| Guaranteed sensitivity | ≤ 100 fM europium in 96 or 384 wells |
| Wavelength range | 300–750 nm |
| Wavelength selection | Monochromators, tunable in 1.0 nm increments |
| Bandwidth (EX, EM) | 9 nm, 15 nm |
| Optimized Precision | ≤ 3.5 mP standard deviation at 1 nM fluorescein in 96 wells |
| Guaranteed Precision | < 5 mP standard deviation at 1 nM fluorescein in 96 wells |
| Dimensions | 22x16x9” |
| Weight | 36 lbs |
| Power | < 420 watts, 100–240 VAC, 3.5 A, 50/60Hz |
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