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BioTek Synergy HT Multi-Mode Microplate Reader with Absorbance, Fluorescence, and Luminescence Detection
Product Description
The BioTek Synergy HT Microplate Reader is a versatile multi-detection system designed for absorbance (UV/Vis), fluorescence, and luminescence measurements critical in molecular diagnostics, biotechnology, and bioprocessing research. Featuring a dual optic design with separate fluorescence and absorbance channels, it accommodates standard plate formats ranging from 6 to 384 wells and PCR tubes up to 1.25″ in height, making it essential for applications like gene editing workflows and biomanufacturing.
Key technical specifications include:
- Absorbance Detection: Monochromator wavelength range of 200–999 nm adjustable in 1 nm increments, 2 photodiode detectors, dynamic range of 0–4.0 OD with 0.001 OD resolution, ideal for quantitative UV/Vis assays and spectral scanning.
- Fluorescence Detection: Deep blocking filters provide excitation from 300–650 nm and emission from 300–700 nm, with a photomultiplier tube (PMT) detection system and sensitivity down to 5 pM fluorescein, suitable for fluorescence analysis in live-cell imaging and synthetic biology research.
- Luminescence Detection: Low-noise PMT detecting wavelengths 300–700 nm with high sensitivity (30 amol ATP typical), supporting molecular diagnostics and bioluminescence assays.
- Temperature Control: Precise incubation from 4°C above ambient to 50°C (±0.5°C at 37°C) allows uniform kinetic assays critical in enzyme kinetics and cell-based assays.
- Shaking Capability: Built-in low, medium, high, and variable shaking modes support sample mixing for bioprocessing experiments.
- Read Methods: Include kinetic, end point, spectral scanning, and well area scanning with rapid read speeds—96 wells in 31 seconds and 384 wells in 80 seconds—facilitating high-throughput screening in clinical diagnostics and synthetic biology workflows.
- Integration: BioStack-ready for automation compatibility, enhancing throughput in biopharmaceutical production pipelines.
The Synergy HT employs a tungsten halogen lamp for absorbance and a xenon flash lamp optimized for UV/Vis detection. Its robust filter system minimizes excitation-emission light leakage to ensure precise fluorescence measurements. The system is computer-controlled via powerful Gen5 software for streamlined data acquisition and analysis, aiding researchers in biotechnology, cell imaging, and molecular cloning workflows.
Note: The time-resolved fluorescence and dispenser modules are not included with this model.
Overall, the BioTek Synergy HT is a high-performance, multi-mode microplate reader widely adopted in synthetic biology research, molecular diagnostics, and bioprocessing environments requiring reliable and sensitive detection across multiple assay formats.
Item Stats
Product details
| Detection Mode | Absorbance (UV/Vis), Luminescence, Fluorescence |
| Read Method | Kinetic, End Point, Spectral Scanning, Well Area Scanning |
| Read modes | Normal, Rapid and Sweep |
| Temperature Control | 4°C above ambient to 50°C ± 0.5°C at 37↑8C |
| Shaking | Yes, Built-in, Low Medium, High and Variable |
| Plate Formats | 6 to 384 well plates, |
| Wavelength Selection | Monochromator |
| Detector | Photodiode (2) |
| Wavelength Range | 200 – 999nm, 1nm increments |
| Bandpass | 2.4 nm |
| Dynamic Range | 0 – 4.0 OD |
| Resolution | 0.001 OD |
| Monochromator Wavelength Accuracy | ±0.2nm |
| Monochromator Wavelength Repeatability | ±0.2nm |
| Sensitivity | Fluorescein 5 pM typical |
| Wavelength Selection | Deep blocking fliters |
| Excitation Range | 300 – 650 nm |
| Emission Range | 300-700 nm |
| Dynamic Range | 5 decades |
| Detection System | PMT |
| Reading Speed | 96 well at 31 seconds, 384 wells at 80 seconds |
| Sensitivity | 30 amol ATP typical (flash) |
| Wavelength Range | 300 – 700 nm |
| Dynamic Range | > decades |
| Detection system | Low noise PMT |
| Power | 100 – 240 VAC 50/60Hz |
| Dimensions | 16″ x 15″ x 10″ (L x W x H) |
| Weight | 40 lbs |
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