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Molecular Devices SpectraMax i3 Multi-Mode Microplate Reader with Modular Cartridge System
Product Description
The Molecular Devices SpectraMax i3 Multi-Mode Plate Reader is a versatile microplate reader designed for comprehensive assay applications in biotechnology, molecular diagnostics, and biomedical research. Featuring integrated absorbance, fluorescence (top and bottom reading), and luminescence detection modes, this instrument supports a broad dynamic range and advanced modular capabilities via swappable cartridges, making it essential for molecular cloning workflows and widely adopted in synthetic biology research.
Key technical specifications include:
- Wavelength ranges: Absorbance 230–1000 nm, Fluorescence 250–830 nm, Luminescence 300–850 nm
- Light source: Spectral Fusion Illumination combining high-powered LEDs, xenon flash lamp, and laser diodes in detection cartridges
- Detectors: Cooled photomultiplier tube (PMT) and photodiode, enabling enhanced sensitivity and linearity (< ±0.010 OD)
- Reading modes: Endpoint, kinetic, spectral scan, and well scan for various assay formats
- Plate compatibility: Supports 6- to 1536-well plates and cuvettes (via adapter)
- Temperature control: Ambient up to 45°C for incubation during measurements
- Plate shaking: Linear and orbital shaking for homogenization during kinetic assays
- Modular expansions: Optional cartridges for Western Blot, AlphaScreen, AlphaLisa, HTRF, TRF, Fluorescence Polarization, and Dual-Color LUMI detection
- Software: SoftMax Pro provides assay optimization featuring excitation and emission wavelength selection guidance
- Performance: Fluorescence sensitivity optimized to detect as low as 0.5 pM fluorescein (96-well) and 1 pM fluorescein (384-well) plates, supporting critical biopharmaceutical production pipelines
This system is particularly suited for live-cell imaging quantification, fluorescence analysis, and molecular diagnostics assays, providing a flexible solution capable of scaling with evolving research needs. Its modular cartridge design allows laboratories engaged in bioprocessing, gene editing, and synthetic biology applications to adapt without replacing the core instrument, ensuring long-term versatility and cost efficiency.
The SpectraMax i3’s combination of broad wavelength coverage, modular read configurations, and precise temperature and shaking controls makes it a reliable analytical tool for biotechnology labs requiring multi-mode detection across diverse sample types and assay formats.
Item Stats
Product details
| Read modes | Absorbance Fluorescence (top/bottom read) Luminescence (top read) Optional Modes: (cartridge dependent) Scanlater Western blot,AlphaScreen and AlphaLisa, HTRF detection, TRF detection, Fluorescence Polarization, Dual-Color LUMI |
| Wavelength ranges | Abs: 230 – 1000 nm FL: 250 – 830 nm Lumi: 300 – 850 nm |
| Wavelength selection | Cartridge filters and monochromator tunable in 1 nm increments |
| Absorbance photometric accuracy/linearity | < ±0.010 OD ±1.0%, 0 – 3 OD VIS 0 – 2 OD UV |
| Absorbance photometric precision/repeatability | < ±0.003 OD ±1.0%, 0 – 3 OD VIS 0 – 2 OD UV |
| Sensitivity top read optimized | 0.5 pM fluorescein 96-well, 1 pM 384-well |
| Light source(s) | Spectral Fusion Illumination (Xenon flash lamp + high-powered LEDs or laser diode in detection cartridges) |
| Reading Capabilities | Microplates, cuvettes (via adapter) |
| Detector(s) | PMT and/or photodiode |
| Read types (endpoint, kinetic, etc) | Endpoint Kinetic Spectrum scan Well Scan |
| Read times | Abs: 30 seconds FL: 25 seconds Lumi: 30 seconds |
| Plate shaking | Linear Orbital |
| Plate type(s) | 6-1536 well plates |
| Temperature control | Ambient + 4°C to 45°C |
| Dimensions | 16x24x18” |
| Weight | 69lbs |
| Power | 100-240vac, 2A, 50/60Hz |
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