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Agilent Advanced Analytical Fragment Analyzer 5300 for DNA/RNA Size and Quality Analysis

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Product Description

The Agilent Advanced Analytical Fragment Analyzer 5300 is a fluorescence-based capillary electrophoresis instrument designed for precise nucleic acid analysis in molecular biology and biotechnology research. It supports the sizing, separation, and quantification of DNA, RNA, and PCR fragments with high resolution and sensitivity, essential for applications such as next-generation sequencing (NGS) library preparation, molecular diagnostics, and gene editing workflows.

Key technical features include:

  • Fluorescence detection using intercalating dyes for sensitive measurement of nucleic acid fragments.
  • Compatibility with 48- and 96-capillary arrays, enabling flexible medium to high sample throughput (up to 240 samples).
  • Minimum injection volume of 25 µL with actual sample requirements as low as 2.5 µL, conserving precious genetic materials.
  • High sizing accuracy with resolution down to 3 base pairs for fragments under 300 bp, critical for applications requiring fine differentiation of nucleic acid size.
  • Capacity for three sample trays, each holding a 96-well PCR microplate, supporting large-scale screening and quality control.
  • Applications tailored to NGS library quality assessment, genomic DNA fragmentation analysis, cell-free DNA monitoring, and RNA integrity evaluations in molecular diagnostics and bioprocessing workflows.

The instrument includes user-friendly Fragment Analyzer ProSize software for automated data acquisition, analysis, and reporting. It provides controlled sample handling with precise plate and pump movements ensuring reproducibility. The device operates on a standard 100-240 VAC power supply and fits benchtop laboratory environments.

This fragment analyzer is widely adopted among molecular biology researchers, biotechnologists, and clinical diagnostics labs for workflows demanding rapid, accurate nucleic acid sizing, making it essential in the fields of synthetic biology, biomanufacturing, and molecular cloning validation.

Comprehensive servicing and quality testing have been performed, verifying key systems including power stability, software communication, pump and plate operation, optical alignment, pressurization, and high voltage control.

This system facilitates high-throughput, reliable analysis, streamlining nucleic acid characterization critical to molecular diagnostics, gene editing validation, and bioprocessing pipelines.


Keywords: fragment analyzer, capillary electrophoresis, DNA sizing, RNA analysis, nucleic acid quantification, next-generation sequencing library QC, molecular diagnostics instrument, fluorescence-based fragment analysis, biotechnology research equipment, biomanufacturing quality control, synthetic biology nucleic acid analysis.


Item Stats

Status
For Sale
State
used
Brand
Advanced Analytical
Model
Advanced Analytical Fragment Analyzer 5300
Location
Walpole - United States

Product details

StockIn Stock
BrandAdvanced Analytical
Product CodeSM100786-5300A
ConditionPre-Owned

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