Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader ABS FL TRF LUM

Manufacturer: Molecular Devices

Model: SpectraMax M2e

Description:

The SpectraMax® M2 and M2e systems
from Molecular Devices are multidetection microplate readers with dual monochromators, dual-mode cuvette ports and top and bottom reading capability (top reading only on the M2). Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Endpoint, kinetic, spectrum and area-well scanning reading modes and the PathCheck® sensor enable homogeneous and heterogeneous microplate assays to be performed in a flexible system. SpectraMax M2 readers enable easy conversion and optimisation from very low throughput assays to medium to high throughput assays, faster, more accurate results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be performed with a single read command.

Core features:

• Multi-detection capability
• Top and bottom read (M2e only)
• Dual-mode cuvette connection
• Two monochromators
• PathCheck and well volume sensor
• Validation of device and software
• Robot compatible

Technical data:

Absorbance photometric performance

Wavelength selection: Monochromator tunable in 1 nm steps
Wavelength range: 200-1000 nm
Wavelength bandwidth: ≤ 4.0 nm
Wavelength accuracy: ±2.0 nm
Repeatability of the wavelength: ±0.2 nm
Photometric range: 0-4.0 OD
Photometric resolution: 0.001 OD
Photometric accuracy (microplate): < ±0.006 OD ±1.0%, 0-2 OD
Photometric accuracy (cuvette): < ±0.005 OR ±1.0%, 0-2 OD
Photometric accuracy: < ±0.003 OD ±1.0%, 0-2 OD
Flatness of the baseline: < 0.001 OD
Stray light: < 0.05% at 230 nm

Fluorescence photometric performance

Dual monochromators: step size of 1 nm
EX wavelength range: 250-850 nm
EM wavelength range: 360-850 nm
Wavelength bandwidth (EX, EM): 9 nm
Optimised top-read sensitivity: 5 pM (fluorescein) in 96 wells
Top-read guaranteed*sensitivity: 15 pM (fluorescein) in 96 wells

Time-resolved fluorescence (secondary mode)

EX wavelength range: 250-850 nm
EM wavelength range: 360-850 nm
Data acquisition: 50-1450 µsec. in 200 µsec. steps
Guaranteed sensitivity: 7 pM Eu chelate

Luminescence (secondary mode) 

Wavelength range (M2): 360-850 nm
Guaranteed sensitivity: 50 fM alkaline phosphatase

General photometric performance 

Plate formats: 6, 12, 24, 48, 96, 384 wells
Light source: Xenon flash lamp (1 joule/flash)
Detector: Photomultiplier tube (PMT)
Reading time**96 wells: 384 wells: Abs 18 sec, FI 15 sec Abs 49 sec, FI 45 sec
Shaking time: 0 to 999 seconds
Temperature control: Ambient temperature +4°C to 45°C
Temperature uniformity: < 1°C at 37°C setpoint
Temperature accuracy: ±1°C at 37°C setpoint

General specifications

Power consumption: < 125 watts
Power: 100-240 Vac, 3 A, 50/60 Hz
Dimensions: 23x16x9 cm
Weight: 35lbs

Scope of delivery:

• SpectraMax M2e
• Operating instructions as PDF via e-mail

The device is in absolute top condition and has been 100% tested for function.