Invitrogen MPK5000 Neon Transfection System

The Neon Transfection System by Invitrogen facilitates rapid and efficient nucleic acid delivery into all mammalian cell types, including primary, stem, and especially challenging-to-transfect cells. This versatile and open system enables high-quality transfections using user-defined or optimized protocols in three straightforward steps, with as few as 20,000 cells per reaction. The revolutionary reaction chamber significantly enhances transfection efficiency and cell viability. The system is efficient, achieving up to 90% success in various cell types; flexible, accommodating 20,000 to 6,000,000 cells per reaction; simple, with a single reagent kit for all cell types; and versatile, allowing for unrestricted optimization of electroporation parameters. Specifications include a format of electroporation pipette tip, a power limit of 300 W, a current limit of 3.0 – 6.0 A, a reaction time of 1 second, and a voltage limit of 0.5 – 2.5 kV. It is classified as animal origin-free, reduced serum, chemically defined, low-protein, reduced growth factors, and xeno-free. Compatible with transfection-grade nucleic acid purification kits, siRNA vectors, and DNA vectors, its sample loading volume and starting material requirements vary by kit and cell type, accommodating 20,000 to 6,000,000 cells.