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Invitrogen MPK5000 Neon Transfection System
Description from the supplier
The Invitrogen MPK5000 Neon Transfection System is a versatile, second-hand instrument designed to facilitate the rapid and effective delivery of nucleic acids into a wide range of mammalian cell types, including primary, stem, and cells traditionally considered difficult to transfect. This adaptable system allows researchers to achieve superior transfection outcomes through both optimized and custom protocols, executed in just three straightforward steps. Capable of processing as few as 2 x 10^4 cells per reaction, the advanced reaction chamber significantly enhances both transfection efficiency and cell viability.
Key Features:
- **Efficiency:** Achieves transfection rates of up to 90% in a variety of cell types, including those that are challenging to transfect, such as primary and stem cells.
- **Flexibility:** Facilitates transfection across a broad range, accommodating from 2 x 10^4 to 6 x 10^6 cells per reaction.
- **Simplicity:** Streamlined operation with a single reagent kit applicable to multiple cell types.
- **Versatility:** Open system design with customizable electroporation parameters.
Specifications:
- **Format:** Electroporation Pipette Tip
- **System:** Neon Transfection System
- **Compatible Cell Types:** Established Cell Lines, Hard-to-Transfect Cells, Stem Cells, Primary Cells
- **Power Limit:** 300 W
- **Current Limit:** 3.0 – 6.0 A
- **Reaction Time:** 1 second
- **Voltage Limit:** 0.5 – 2.5 kV
- **Classification:** Animal Origin-Free, Reduced Serum, Chemically Defined, Low-Protein, Reduced Growth Factors, Xeno-Free
- **Serum Compatibility:** Yes
- **Compatible Products:** Transfection Grade Nucleic Acid purification kits, siRNA vectors, DNA vectors
- **Sample Loading Volume:** Variable, dependent on the kit used
- **Sample Type (Specific):** Plasmid DNA, Synthetic siRNA
- **Purity or Quality Grade:** Transfection Grade
- **Starting Material (Cell #):** 2 x 10^4 – 6 x 10^6 cells, varies by cell type
This system is an excellent choice for researchers seeking reliable and reproducible transfection results across a wide array of experimental conditions.
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