Bio-Techne ProteinSimple Wes Automated Simple Western Assay System

A Simple Western size assay is an automated Western – no gels, no transfer devices, no blots, no film and no manual analysis.

Simple Westerns take place in a capillary. Wes automates all steps of the process for you including sample loading, protein separation, immunoprobing or labeling, washing, detection and data analysis. Up to 25 samples can be processed in a single run.

Wes performs Simple Western size-based assays: Immunoassay and Total Protein assay. All you have to do is pipette your samples and immunoreagents into Wes’s pre-filled plate, load the capillary cartridge and press start.

Sample Required Total Protein: 0.3-1.2 µg lysate

Sample Required Immunoassay Charge: 0.6-1.2 µg

Volume Required Total Protein: 3 µL/well

Volume Required Immunoassay Charge: 3 µL/well

Size or pI Range Total Protein: Molecular weight (MW) ladders range from 2-440 kDa

Size or pI Range Immunoassay Charge: Molecular weight (MW) ladders range from 2-440 kDa

Sizing CV Total Protein: <10%

Sizing CV Immunoassay Charge: <10%

Intra-assay CV Total Protein: <15%

Intra-assay CV Immunoassay Charge: <15%

Resolution (± percent difference in MW) Total Protein: ± 15-20% for MW <20 kDa, ±10 % for MW > 20 kDa

Resolution (± percent difference in MW) Immunoassay Charge: ± 15-20% for MW <20 kDa, ±10 % for MW > 20 kDa

Quantitation CV Total Protein: <20%

Quantitation CV Immunoassay Charge: <20%

Dynamic Range Total Protein: 2-3 logs

Dynamic Range Immunoassay Charge: 3 logs

Sensitivity Total Protein: ng

Sensitivity Immunoassay Charge: Low pg

Capillary: 5 cm, 100 µm, 400 nL

Dimensions (HxWxD): 360 x 360 x 570 mm

Weight: 23 kg

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