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Brand
System
Supplier
LabMakelaar Benelux B.V.
Location
Zevenhuizen - Netherlands
Price
€14,500.00 
(ex. VAT)
This item is
for sale
State of item
used
Categories

Bio-Techne ProteinSimple Wes Automated Simple Western Assay System

LabMakelaar Benelux B.V.€14,500.00 (ex VAT)
item image

Description by the supplier

A Simple Western size assay is an automated Western – no gels, no transfer devices, no blots, no film and no manual analysis.


Simple Westerns take place in a capillary. Wes automates all steps of the process for you including sample loading, protein separation, immunoprobing or labeling, washing, detection and data analysis. Up to 25 samples can be processed in a single run.


Wes performs Simple Western size-based assays: Immunoassay and Total Protein assay. All you have to do is pipette your samples and immunoreagents into Wes’s pre-filled plate, load the capillary cartridge and press start.


Sample Required Total Protein: 0.3-1.2 µg lysate


Sample Required Immunoassay Charge: 0.6-1.2 µg


Volume Required Total Protein: 3 µL/well


Volume Required Immunoassay Charge: 3 µL/well


Size or pI Range Total Protein: Molecular weight (MW) ladders range from 2-440 kDa


Size or pI Range Immunoassay Charge: Molecular weight (MW) ladders range from 2-440 kDa


Sizing CV Total Protein: <10%


Sizing CV Immunoassay Charge: <10%


Intra-assay CV Total Protein: <15%


Intra-assay CV Immunoassay Charge: <15%


Resolution (± percent difference in MW) Total Protein: ± 15-20% for MW <20 kDa, ±10 % for MW > 20 kDa


Resolution (± percent difference in MW) Immunoassay Charge: ± 15-20% for MW <20 kDa, ±10 % for MW > 20 kDa


Quantitation CV Total Protein: <20%


Quantitation CV Immunoassay Charge: <20%


Dynamic Range Total Protein: 2-3 logs


Dynamic Range Immunoassay Charge: 3 logs


Sensitivity Total Protein: ng


Sensitivity Immunoassay Charge: Low pg


Capillary: 5 cm, 100 µm, 400 nL


Dimensions (HxWxD): 360 x 360 x 570 mm


Weight: 23 kg

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