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A Simple Western size assay is an automated Western – no gels, no transfer devices, no blots, no film and no manual analysis.
Simple Westerns take place in a capillary. Wes automates all steps of the process for you including sample loading, protein separation, immunoprobing or labeling, washing, detection and data analysis. Up to 25 samples can be processed in a single run.
Wes performs Simple Western size-based assays: Immunoassay and Total Protein assay. All you have to do is pipette your samples and immunoreagents into Wes’s pre-filled plate, load the capillary cartridge and press start.
Sample Required Total Protein: 0.3-1.2 µg lysate
Sample Required Immunoassay Charge: 0.6-1.2 µg
Volume Required Total Protein: 3 µL/well
Volume Required Immunoassay Charge: 3 µL/well
Size or pI Range Total Protein: Molecular weight (MW) ladders range from 2-440 kDa
Size or pI Range Immunoassay Charge: Molecular weight (MW) ladders range from 2-440 kDa
Sizing CV Total Protein: <10%
Sizing CV Immunoassay Charge: <10%
Intra-assay CV Total Protein: <15%
Intra-assay CV Immunoassay Charge: <15%
Resolution (± percent difference in MW) Total Protein: ± 15-20% for MW <20 kDa, ±10 % for MW > 20 kDa
Resolution (± percent difference in MW) Immunoassay Charge: ± 15-20% for MW <20 kDa, ±10 % for MW > 20 kDa
Quantitation CV Total Protein: <20%
Quantitation CV Immunoassay Charge: <20%
Dynamic Range Total Protein: 2-3 logs
Dynamic Range Immunoassay Charge: 3 logs
Sensitivity Total Protein: ng
Sensitivity Immunoassay Charge: Low pg
Capillary: 5 cm, 100 µm, 400 nL
Dimensions (HxWxD): 360 x 360 x 570 mm
Weight: 23 kg
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