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Waters ACQUITY UPLC I-Class Ultra-High-Pressure Liquid Chromatography System with PDA and FLR Detectors


Product Description

The Waters ACQUITY UPLC I-Class System with PDA & FLR Detectors is a precision-engineered ultra-high-pressure liquid chromatography (UPLC) platform designed for advanced chromatographic separation and analysis in biotechnology, pharmaceutical research, and molecular diagnostics. Built to support sub-2 µm particle columns and operate at pressures up to 18,000 psi, the system delivers enhanced chromatographic resolution, sharper peak shapes, and rapid separations essential for complex and low-concentration sample workflows.

Key technical features include:

  • Binary Solvent Manager enabling precise isocratic and gradient elution with ballistic gradient capability for accelerated cycle times and reproducible method execution.
  • Flow-Through-Needle Sample Manager (FTN) offering high injection precision, low carryover, and wide injection volume flexibility with optional auto-dilution for enhanced sample recovery and sensitivity.
  • Active Column Heating (CH-A) with mobile phase preheating ensures consistent thermal control, improving retention time reproducibility and reliable method transfer between labs.
  • Photodiode Array (PDA) Detector with a UV/Vis wavelength range of 190-500 nm provides full spectral data acquisition, supporting compound identification, peak purity evaluation, and quantitative analysis.
  • Fluorescence (FLR) Detector optimized for trace-level detection using excitation wavelengths from 200-890 nm and emission from 210-900 nm, delivering excellent signal-to-noise ratio for native or derivatized fluorescent compounds.
  • Optimized low dead-volume fluidics reduce band broadening, enhancing detection sensitivity and resolution in demanding analytical workflows such as gene editing monitoring, bioprocessing quality control, and synthetic biology research.

This UPLC system, widely used in biomedical engineering and molecular diagnostics, supports rigorous analytical protocols requiring consistent, reproducible results over extended sequences. Integrated features such as intelligent solvent delivery with vacuum degassing and automated priming minimize maintenance requirements while ensuring system stability.

The ACQUITY UPLC I-Class System is fully compatible with Waters Empower Software, facilitating streamlined data acquisition, advanced instrument control, and comprehensive analysis for workflows in biotechnology, clinical diagnostics, and environmental testing.

Overall, this system offers a robust and versatile solution essential for high-resolution chromatography in the development of biopharmaceuticals, molecular cloning workflows, and complex chemical separation challenges where analytical confidence and method robustness are critical.


Item Stats

Status
For Sale
State
used
Brand
Waters
Model
ACQUITY UPLC I-Class System w/ PDA & FLR Detectors
Location
Hampton - United States
Shipping Info
3 to 5 Business Days

Product details

PDA DetectorSpecification
Wavelength Range190 to 500 nm
Optical Resolution1.2 nm
Digital Resolution1.2, 2.4, 3.6, 4.8, 6.0, 7.2, 8.4, 10.8, 12.0
Wavelength Accuracy± 1.0 nm
Wavelength Repeatability± 0.1 nm
Digital FilterVariable with data rate
Order FilterFixed, 340 to 500 nm
Noise (Shunt in Place of Flow Cell)1 - 400 nm
Noise (10 mm Analytical Flow Cell)4 nm
Drift (Medium Shunt Cell and 10 mm Analytical Flow Cell)~ 0.5 nm
Linearity< 5% at 2.0 AU, propylparaben series at 257 nm
Data Rate1, 2, 5, 10, 20, 40, and 80
FLR DetectorSpecification
Wavelength Range200 to 890 nm (Excitation) 210 to 900 nm (Emission)
Bandwidth20 nm
Wavelength Accuracy± 3 nm
Wavelength Repeatability± 0.25 nm
SensitivitySignal-to-noise > 1000 (Raman spectrum of water)
Measurement Range0.001 to 10,000 EU
Data Acquisition RateUp to 80Hz
Unattended OperationLeak sensor, full 96-hour diagnostic data displayed through ACQUITY UPLC console software
Light SourceHg/Xe arc lamp
Flow Cell DesignAxially illuminated
Cell Volume< 2 uL
Pressure Limit3447 kPa (34 bar, 500 psi) for standard flow cell
MaterialsStainless steel, fused silica, FEP, PEEK

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