Thermo PikoReal 96 Real-Time PCR Thermal Cycler with 5 Optical Channels for Multiplexing

The Thermo PikoReal 96 Real-Time PCR System is a high-precision thermal cycler designed for reliable quantitative PCR (qPCR) and multiplex gene expression analysis. Featuring a superior thermal block design with a temperature range of 4 to 99.9°C and an accuracy of ±0.2°C, it supports rapid heating rates exceeding 5°C/sec and cooling at 4.5°C/sec. The system incorporates five optical channels spanning excitation wavelengths from 475 to 640 nm and detection from 520 to 740 nm, enabling multiplex amplification of up to 4 target genes simultaneously.

This thermal cycler operates as a standalone unit or via PC-controlled flexible PikoReal software, allowing real-time experiment monitoring and USB protocol management. Its compact design dramatically reduces plastic waste and reagent consumption, making it a cost-effective solution for molecular diagnostics and bioprocessing workflows. The Ethernet interface supports connecting up to 10 instruments to a single PC, facilitating high-throughput setups critical in synthetic biology and molecular cloning applications.

Key functionalities include absolute and relative quantification modes, high-resolution melt (HRM) analysis, allelic discrimination, and melt curve analysis. The system’s precise temperature control and multiplex capabilities make it essential for advanced biotechnology research, including gene expression profiling, molecular diagnostics, and biopharmaceutical production pipelines.

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This unit is manufactured in the USA and verified for full functionality despite minor cosmetic imperfections. It is widely adopted in clinical diagnostics, synthetic biology research, and live-cell fluorescence analysis, offering researchers a precise and efficient platform for qPCR applications.