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Thermo Scientific PikoReal 24 Real-Time PCR System with Multiplex qPCR and 24-Well Block

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Product Description

The Thermo Scientific PikoReal 24 Real-Time PCR System is a specialized quantitative PCR thermal cycler tailored for low sample throughput applications with its 24-well format block. This system supports advanced multiplex PCR workflows critical in molecular diagnostics, gene editing assays, and biotechnology research including molecular cloning and synthetic biology.

Key technical features include:

  • Thermal block: 24 wells compatible with low profile strip tubes and PCR tubes, accommodating sample volumes from 10 to 50 μl.
  • Temperature control: High precision with ±0.2°C accuracy and uniformity up to ±0.15°C; heating rates >5°C/sec and cooling rates 4.5°C/sec enable efficient cycle times.
  • Heated lid system: Automatic temperature control (50–110°C) and pressing setting ensure condensation prevention and sample integrity.
  • Optical detection: Utilizes a CCD detector paired with 5 excitation LEDs covering 475–640 nm excitation range and 520–740 nm emission range, supporting up to 5 detection channels for hydrolysis probe chemistries such as 5’Nuclease/TaqMan assays.
  • Multiplexing capacity: Up to 4 targets can be simultaneously detected, enabling complex gene expression and allelic discrimination studies necessary in biopharmaceutical and synthetic biology pipelines.
  • Analysis modules: Features absolute and relative quantification, melting curve analysis, allelic discrimination, and High Resolution Melting (HRM) analysis using robust algorithms that do not require external reference dyes.
  • Control and software: Offers standalone operation with USB protocol uploads or PC control via PikoReal software allowing remote online monitoring for streamlined real-time data acquisition and experiment management.

Compact and lightweight (9.2 kg; 300 x 230 x 310 mm), the PikoReal 24 Real-Time PCR System is engineered for precision in bioprocessing, clinical diagnostics, and biomanufacturing workflows, widely adopted in live-cell gene expression profiling and fluorescence-based molecular assays. Its combination of thermal accuracy, sensitivity (down to 1 copy detection), and multiplex capacity make it an essential instrument for cutting-edge biotechnology research and molecular biology laboratories focused on molecular diagnostics and genetic analysis.


Item Stats

Status
For Sale
State
used
Brand
Thermo Scientific
Model
PikoReal 24 Real Time PCR System
Location
Zevenhuizen - Netherlands

Product details

Weight0,0 kg
BrandThermo (Scientific)
Warranty6 months
ConditionUsed, in good condition
Date of Manufacture2013

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