Roche LightCycler 96 Real-Time PCR System with PC Model 5815916001

The Roche LightCycler 96 Real-Time PCR System (Model 5815916001) is a high-sensitivity molecular diagnostics instrument designed for rapid and accurate quantitative PCR analysis. This thermal cycler supports 96 samples simultaneously and utilizes a Peltier element for precise temperature control with ±0.2 °C accuracy and a maximum heating speed of 4.4 °C/sec. It features advanced gradient control up to 20 °C within the 37–98 °C range, enabling multiplex PCR assays for complex bioprocessing applications.

Key technical features include a high-power broad spectrum LED excitation source for four fixed wavelength channels (470, 533, 577, 645 nm) and corresponding detection wavelengths (514, 572, 620, 697 nm), allowing fluorescence detection of common dyes such as SYBR Green I, FAM, VIC, and Cy5. The instrument's fluorescence detection system employs glass fiber optics and CCD cameras, eliminating the need for ROX normalization, which enhances reproducibility and sensitivity in gene expression and molecular diagnostics workflows.

The unit supports intercalator dye and hydrolysis probe assays and offers 4-color multiplexing capabilities essential for complex synthetic biology and biomanufacturing experiments. The included PC and Windows-compatible software facilitate comprehensive data analysis modules such as absolute and relative quantification, Tm calling, endpoint genotyping, and high-resolution melting (HRM) analysis.

Physically, it measures 40×40×53 cm (W×D×H) and weighs approximately 27 kg. The system is ideal for molecular biology researchers focused on biotechnology, gene editing validation, infectious disease analysis, and molecular diagnostics in clinical and research settings. This used unit is sold as-is with warranty coverage for defects reported within 10 days of receipt.

Widely used in biopharmaceutical production pipelines, this LightCycler 96 provides reliable and rapid qPCR performance critical for various applications including gene expression analysis, gene editing validation, and bioprocess monitoring.