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Roche Diagnostics LightCycler 96 Real-Time PCR System LC96 IP
Product Description
The Roche Diagnostics LightCycler 96 is a used 96-well real-time PCR system for quantitative PCR, multiplex fluorescence analysis, endpoint genotyping, high-resolution melting, and qualitative detection. It supports standalone touchscreen operation, USB data transfer, and LAN connectivity without requiring a dedicated PC for instrument operation.
Key specifications
- Throughput: 96 samples per run
- Reaction volume: 10–50 µL per well
- Reaction vessels: 8-strip tubes and 96-well plates
- Reaction block: 0.1 mL format
- Reaction time: 40 minutes or less for 3-step, 40-cycle runs, depending on the enzyme
- Temperature range: 37–98 °C
- Heating rate: 4.4 °C/s maximum
- Cooling rate: 2.2 °C/s maximum
- Temperature accuracy: ±0.2 °C
- Temperature uniformity: ±0.4 °C
- Gradient control: 37–98 °C with a maximum 20 °C gradient span
- Detection: Four-color fluorescence detection using glass-fiber excitation/detection and a CCD camera
- Excitation wavelengths: 470, 533, 577, and 645 nm
- Detection wavelengths: 514, 572, 620, and 697 nm
- Multiplexing: Four-color assays with fixed excitation/detection combinations
- Compatible chemistries: SYBR Green I and other intercalating dyes, hydrolysis probes, and HRM workflows
- Noise level: 43 dB or less
- Power: AC 100–125 V ±10%, 600 W, 50/60 Hz
- Dimensions: 400 × 400 × 530 mm
- Weight: Approximately 27 kg
Supported applications include quantitative gene expression, molecular diagnostics, biotechnology research, synthetic biology workflows, endpoint genotyping, and fluorescence-based nucleic acid analysis. The used unit is offered as-is, and accessories are limited to those stated or shown in the listing.
Item Stats
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Ph.D. Hsin-Wen Liang
Northeastern UniversityFrom €15,407.63*ex. VAT
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