Offering the best quotes for your research!
New and used scientific instruments
🚚 Shipping Worldwide 🌎
✉️ info@questpair.com
Qiagen Rotor-Gene Q 5Plex Real-Time PCR System with Rotary Thermal Cycling
Product Description
The Qiagen Rotor-Gene Q 5Plex Real-Time PCR System is a precision rotary thermal cycler designed for quantitative PCR workflows requiring exceptional temperature uniformity and multiplex detection performance. Utilizing a unique spinning rotor mechanism, it eliminates edge effects and well-to-well variation common in block-based qPCR instruments, ensuring reproducible amplification results, critical in molecular diagnostics and bioprocessing research.
Key specifications include:
- Thermal Cycling: Temperature accuracy ±0.5ºC, resolution ±0.02ºC, range 35–99ºC, with rapid ramp rates >15ºC/s heating and >20ºC/s cooling for efficient cycling.
- Optical Detection: Supports up to 6 fluorescence channels from UV to near-infrared, employing high-energy LEDs and photomultiplier detectors for sensitive multiplex qPCR assays, including 5-plex detection capability.
- Sample Capacity: Accommodates up to 100 samples per run using Rotor-Disc 100 format and is compatible with 0.1 ml tubes and strip tubes, ideal for medium-throughput molecular biology and clinical applications.
- Dynamic Range: 10 orders of magnitude, enabling detection from low to high template concentrations, essential for gene expression studies, mutation analysis, and pathogen detection.
- Environmental and Power: Operates at 18–30ºC with 10–75% noncondensing humidity; power requirements 100–240 V AC, 50–60 Hz.
This benchtop system is widely adopted in biotechnology, molecular diagnostics, synthetic biology, and biomanufacturing workflows, where reliable multiplex PCR and quantitative reproducibility are paramount. The Rotor-Gene Q is essential for labs conducting gene expression analysis, copy number variation studies, and pathogen genotyping. It supports validated assays, enabling seamless integration into existing PCR workflows without extensive revalidation or retraining.
Included accessories enhance operational readiness, such as a Rotor with Locking Cap (72 x 0.1 ml tubes), Windows 10 notebook with Rotor-Gene Q Pure Detection Software, communication cables, and power cords. This system provides a compact footprint and established performance metrics, making it a preferred choice for researchers and clinical scientists seeking consistent, high-throughput real-time PCR instrumentation.
Item Stats
Product details
| Dynamic range | 10 orders of magnitude |
| Heat dissipation/thermal load | Average, 0.183 kW (632 BTU/hour); Peak, 0.458 kW (1578 BTU/hour) |
| Humidity | 10–75% (noncondensing) |
| Operating temperature | 18-30ºC (64-86ºF) |
| Optical System | Up to 6 channels spanning UV to infra-red wavelengths |
| Excitation sources | High energy light-emitting diodes |
| Green | 470 ± 10 excitation (nm) 510 ± 5 Detection (nm) |
| Yellow | 530 ± 5 excitation (nm)557 ± 5 Detection (nm) |
| Orange | 585 ± 5 excitation (nm)610 ± 5 Detection (nm) |
| Red | 625 ± 5 excitation (nm)660 ± 5 Detection (nm) |
| Crimson | 680 ± 5 excitation (nm)712 ± 5 Detection (nm) |
| Detector | Photomultiplier |
| Acquisition time | 4 seconds |
| Samples per run | Tubes 0.2 ml; Strip Tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; Up to 100 samples per run using a Rotor-Disc 100 |
| Temperature range | 35 to 99ºC |
| Temperature accuracy | ±0.5ºC (type, measured 30 seconds after clock start) |
| Temperature resolution | ±0.02ºC (smallest programmable increment) |
| Temperature uniformity | ±0.02ºC; Ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling |
| Typical run time | 40 cycles in 45 min with the QIAGEN RG Kits (assay dependent) |
| Power | 100–240 V AC, 50–60 Hz, <520 VA (peak) |
| Dimensions | 16″ x 14″ x 11″ inches |
| Weight | 28 lbs |
About QuestPair
Our Mission
QuestPair is Europe's leading platform for scientific equipment, connecting researchers and organizations with verified suppliers of both new and pre-owned laboratory instruments in the biotech and material science industry. Our aim is to make the procurement of scientific instruments more efficient, cost-effective, and reliable, so that laboratories can focus on advancing science rather than searching equipment and negotiating deals.
Why Choose Us
Founded by scientists for scientists, we understand your challenges. Our AI-powered platform offers transparent pricing, verified quality, and expert support, ensuring you find the perfect equipment for your research needs.
Verified Quality
Every piece of equipment undergoes thorough verification by our expert team, ensuring reliability and performance.
Cost Efficiency
Access both new and premium pre-owned equipment, saving up to 40% without compromising on quality.
Expert Support
Our dedicated team provides personalized guidance throughout your equipment procurement journey.
Ready to Transform Your Research?
Join thousands of biotech scientists who trust QuestPair for their equipment needs.
What customers think about QuestPair
Brilliant service. Everything arrived and was exactly as expected
Jon Welburn
Great customer service! Got me great options for the equipment I was looking for and in a timely manner.
Pamela
Space Lab Technologies, LLCHarm is very responsive to help me find the right equipment. The product I received is in a good condition.
Ph.D. Hsin-Wen Liang
Northeastern UniversityPrice on Request
In Stock
Get a quote within one business day
Verified equipment
Trusted warranties & returns
Safe payment and fast delivery
Didn't find what you're looking for?
Our sales team specializes in sourcing custom and hard-to-find products to meet your exact specifications. Submit a request for a tailored quote.
Frequently Asked Questions
Is this equipment new or refurbished?
How long does shipping take?
What about warranty and returns?
Why request a quote?
Need help choosing the right tool?
- Accurate policy information specific to your chosen product
- Clear understanding of warranty coverage and terms
- Detailed return policy explanation
- Transparent shipping options and costs
Customer Service
Here to help you with: Product Inquiries, Shipping & Support, Technical Support, Business Inquiries and Press.
We are available to assist you Mon-Fri, 10am - 5pm CET.