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Applied Biosystems StepOne Real-Time PCR System for qPCR and Genotyping
Product Description
The Applied Biosystems StepOne Real-Time PCR System is a quantitative PCR (qPCR) instrument designed for robust amplification and real-time fluorescence detection. Featuring a 48-well format and capable of processing reaction volumes from 10–30 µL, this system supports both standard and fast PCR protocols with run times under 2 hours (standard) and 40 minutes (fast mode).
Key technical features include:
- LED-based optical system with 3 emission filters and photodiode detection optimized for TaqMan probe-based assays (FAM, VIC) and SYBR Green dye chemistries.
- Peltier thermal cycling with a temperature range of 4–100°C, excellent uniformity of ±0.25°C, and rapid ramp rate of 4.6°C/s enables precise temperature control essential for PCR fidelity.
- Support for automated SNP genotype calling, melt curve analysis, and multiplex assays, making it an ideal platform for molecular diagnostics, gene expression profiling, and genotyping workflows.
- Intuitive touchscreen GUI and integrated Design Wizard assist in method setup, facilitating customization of experimental parameters including ramp speeds and quantitation methods.
- PC compatibility with StepOne software allows advanced multi-plot data analysis and export to Excel or PowerPoint, supporting bioinformatics workflows in biotechnology and biomedical research.
- Compact footprint (10 x 19 x 21 inches), weighing 45 lbs, suitable for benchtop use in clinical and research laboratories.
This system's high sensitivity, able to detect down to 1 copy, and a dynamic range spanning 9 logs, make it indispensable for applications including molecular cloning, synthetic biology, bioprocessing, and molecular diagnostics. The StepOne Real-Time PCR System is widely adopted in biotechnology research fields requiring quantitative gene expression analysis and precise genotyping, contributing to advanced biomanufacturing and gene editing projects.
Item Stats
Product details
| Format | 48-well Plate, 0.1 ml Tubes |
| Reaction volume Range | 10-30 µl (Standard curve experiments: 40 µl in standard mode is validated) |
| Optics | LED, 3 emission filters, and photodiode |
| Reaction Speed | Fast, Standard |
| Detection Method | SYBR, Primer-Probe Detection |
| Fast Block | Yes |
| Run Time | <2 hrs⁄run (Standard Mode), <40 min⁄run (Fast Mode) |
| High-throughput Compatibility | Multiplexing |
| Dynamic Range | 9 logs of linear dynamic range |
| Sensitivity | 1 copy |
| Temperature Range | 4-100°C |
| Temperature Uniformity | 0.25°C (35 to 95°C) of setpoint/display temperature |
| Thermal Cycling System | Peltier-based System |
| Peak Block Ramp Rate | 4.6°C⁄s |
| Power | 115V 50/60Hz |
| Dimensions (W x D x H) | 10x19x21” |
| Weight | 45 lbs |
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