Applied Biosystems 7500 Fast Real-Time PCR System for Quantitative Molecular Diagnostics

The Applied Biosystems 7500 Fast Real Time PCR System is a high-performance thermal cycler optimized for quantitative molecular diagnostics and biotechnological applications such as gene expression analysis, synthetic biology, and molecular cloning workflows. Featuring a Peltier-based 96-well block, it enables rapid thermal cycling with a peak heating rate of 5.5°C/sec and sample ramp rates of 3.5°C/sec in fast mode and 1.6°C/sec in standard mode, supporting reaction volumes from 10 to 30 µL.

The instrument incorporates a CCD camera paired with a halogen lamp for fluorescence detection, utilizing 5 excitation and 5 emission filters to accurately detect multiple fluorophores. It comes calibrated for key dyes including FAM/SYBR Green, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5, supporting multiplex assays essential for advanced molecular diagnostics and bioprocessing pipelines. The system optionally uses ROX or other calibrated passive reference dyes to normalize signal variability.

Designed to accommodate 96-well low-profile PCR strip plates or 8-tube strips, the system ensures precise temperature control with a range from 4°C to 99.9°C, an accuracy of ±0.5°C, and uniformity within ±1°C. Its compact footprint (340 x 450 x 490 mm) and weight (34 kg) make it suitable for core laboratories engaged in clinical diagnostics, biotechnology research, and biomanufacturing processes.

This real-time PCR instrument is widely adopted in synthetic biology research and critical in biopharmaceutical production pipelines, offering reliable performance for quantitative PCR assays in neuroscience, cell imaging, and molecular diagnostics requiring fast, accurate, and multiplex-capable thermal cycling technology.