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Eppendorf Mastercycler X50i & X50s Gradient Thermal Cycler Bundle for Advanced PCR Applications
Product Description
The Eppendorf Mastercycler X50i & X50s bundle is a cutting-edge gradient thermal cycler system designed for advanced PCR workflows, high-throughput molecular diagnostics, and scalable biotechnology research environments. This integrated solution combines the X50s master unit's high-resolution touchscreen interface with the X50i eco unit's full gradient cycling capability, enabling streamlined program control and expansion up to nine eco units.
Key technical specifications and features include:
- Temperature range: 4°C to 99°C block temperature with a heated lid adjustable between 37°C and 110°C
- Ramp rates: rapid heating at 10°C/sec and cooling at 5°C/sec for efficient cycling throughput
- Gradient technology: innovative 2D-Gradient functionality allowing simultaneous optimization of annealing and denaturation temperatures over a 30°C spread (30°C to 99°C)
- Thermal uniformity: six independently controlled peltier elements ensure uniform temperature control with ±0.2°C to 0.3°C precision depending on cycling conditions
- Capacity and flexibility: flexlid automatically adapts to various PCR consumables including tubes and plates for versatile experimental setups
- Memory and data handling: X50s supports storage of up to 5,000 programs and 500 folders with USB and Ethernet connectivity for seamless data transfer, updates, and system networking
- Network scalability: use Cyclement Manager software (sold separately) to network and manage up to 50 Mastercycler units for high-throughput bioprocessing and large-scale biomanufacturing workflows
- Compact design: front-to-back ventilation reduces bench footprint and supports quiet, continuous operation in busy lab environments
This dual-unit system is widely adopted in molecular biology, synthetic biology, and clinical diagnostics laboratories, providing precision thermal cycling critical for gene editing, molecular cloning workflows, and biotechnology applications requiring thermal gradient optimization. The Mastercycler X50 series supports essential bioprocessing pipelines by delivering reliable, reproducible results and operational flexibility, making it integral to modern biopharmaceutical production and research laboratories focused on fluorescence analysis and live-cell DNA amplification studies.
Item Stats
Product details
| Thermal Range (Block) | 4°C to 99°C |
| Thermal Range (Lid) | 37°C to 110°C |
| Thermal Accuracy | ± 0.15°C |
| Thermal Uniformity | ± 0.2°C at 20°C to 72°C ± 0.3°C at 72°C to 95°C |
| Temperature Control Modes | Fast, Intermediate, Standard, Safe |
| Ramping Rates | Heating: 10°C/sec Cooling: 5°C/sec |
| Sample Capacity | 96 x 0.1 ml PCR tubes 96 x 0.2 ml PCR tubes 1 x 96-well PCR plate (unskirted, semi-skirted, skirted, and low-profile) |
| Memory | Up to 5,000 programs and 500 folders |
| Gradient Spread | 30°C maximum |
| Gradient Temperature Range | 30°C to 99°C |
| Interfaces | USB and Ethernet |
| Heating Technology | Six peltier elements |
| Dimensions (W x D x H) | 10.2 x 21.9 x 14.6 in. (27.5 x 43 x 33 cm) |
| Weight | 25.4 lb. (11.5 kg) |
| Electrical Requirements | 100-240V, 50/60Hz, 850W |
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