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Applied Biosystems 7500 Fast Dx 96-Well Real-Time PCR System for Molecular Diagnostics
Product Description
The Applied Biosystems 7500 Fast Dx Real-Time PCR System is an FDA 510(k)-cleared platform designed for high-throughput molecular diagnostics and quantitative gene expression analysis. Featuring a 96-well fast thermal block optimized for 10 µL reaction volumes (range 10–30 µL), it offers precise thermal cycling with a peak block heating rate of 5.5°C/second and temperature uniformity of ±1°C, ensuring consistent amplification performance critical in molecular cloning and bioprocessing workflows.
Key technical features include:
- Multiplex fluorescence detection with a CCD camera and halogen lamp system equipped with five excitation and emission filters, enabling simultaneous quantification of up to five distinct targets per well.
- Robust dynamic detection range spanning nine logs, capable of detecting a single copy of nucleic acid template in a 20 µL reaction with 99.7% confidence, facilitating sensitive molecular diagnostics and synthetic biology research.
- Rapid cycling technology delivers results in under 40 minutes, supporting accelerated biomanufacturing and gene editing applications.
- Integrated SDS v1.4.1 software provides regulated environment support with security, auditing, and electronic signature features essential for compliance in molecular diagnostics laboratories.
- Optimized for in vitro diagnostic (IVD) use with validated performance in DNA quantification and gene expression studies relevant for biomedical engineering and clinical research.
This system’s combination of speed, sensitivity, and multiplex capability makes it widely adopted in biotechnology fields including molecular diagnostics, synthetic biology, and biopharmaceutical production pipelines requiring reliable, high-throughput real-time PCR analysis.
Note: Though discontinued by the manufacturer, the 7500 Fast Dx remains suitable for validated IVD protocols and research applications. Dye calibration is recommended per assay requirements as detailed in the Operator's Manual.
Item Stats
Product details
| Block Configuration | 96-Well Fast (10 to 30 uL reactions, optimized for 10 uL reactions) |
| Sample Format | Fast 96-Well plates (100 uL) or 8-tube strips (100 uL) |
| Temperature Range | 4°C to 99.9°C |
| Temperature Uniformity | ± 1°C |
| Temperature Accuracy | ± 0.5°C |
| Sample Ramp Rate | Fast mode: 3.5°C/sec Standard mode: 1.6°C/sec |
| Peak Block Heating Rate | 5.5°C/second |
| Run Time | < 40 minutes |
| Optical System | CCD camera with halogen lamp excitation; five-exciation and five-emission filters |
| Dynamic Range | 9 logs of linear dynamic range |
| Sensitivity | Detection of 1 copy of template in 20 uL reaction for a single reporter TaqMan assay, with 99.7% confidence |
| Multiplexing | Up to 5 targets per well |
| Dye Compatibility | FAM/SYBR Green, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5 (Calibration for new dyes within the wavelength range is possible by following the custom dye calibration procedure in the user’s manual) |
| Passive Reference Dyes | ROX dye or any calibrated dye (use of a passive reference dye is optional) |
| Dimensions (W x D x H) | 13.9 x 17.7 x 19.3 in. (34 x 45 x 49 cm) |
| Weight | 75 lb. (34 kg) |
| Electrical Requirements | 100-240V, 50/60Hz, 1080W |
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