Agilent G2539A DNA Microarray Scanner with SureScan Technology

The Agilent G2539A DNA Microarray Scanner, featuring SureScan High-Resolution technology, is essential for high-precision microarray applications requiring extensive genome coverage. It facilitates accurate data acquisition for gene expression, array comparative genomic hybridization (CGH), and miRNA profiling.

The scanner offers an expansive dynamic range across 6 orders of magnitude and incorporates a distinctive autofocus feature that maintains optimal focus throughout the array. This system compensates for any glass aberrations or curvatures, ensuring top-tier data quality. Designed for seamless integration, all system components perform optimally when used alongside Agilent microarrays, providing exceptional sensitivity and data fidelity.

Key features include dynamic range capabilities of 10⁴ in 16-bit format, 10⁵ in 20-bit format, and 10⁶ with XDR scanning. The dynamic auto-focus adjusts focus consistently, and the autoloader allows for hands-free operation with a 48-slide capacity. An integrated barcode reader supports multiple code types while the system accommodates a range of compatible dyes, such as Cyanine 3, Cyanine 5, Alexa 647, Alexa 555, and Alexa 660.

Equipped with a SHG-YAG laser at 532nm and a Helium-Neon laser at 633nm, the scanner maximizes a scan window of up to 21.6 mm x 71 mm. Signal levels are adjustable from 100% to 1%, with a detection limit of just 0.05 chromophores per square micron. Precision is paramount with a pixel placement error of only 1 pixel at 5-micron resolution, and the system maintains a 5% CV global non-uniformity. Scans are quick, achieving 2-color data acquisition within 15 minutes for 3-micron scans and 20 minutes for 2-micron scans across a 61 mm x 21.6 mm area.

Compact with dimensions of 550 x 900 x 635 mm, and a weight of 98 kg, the Agilent G2539A scanner is an exemplary choice for laboratories requiring precise and reliable microarray scanning.