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Lonza 4D Nucleofector Modular Electroporation System for Cell Transfection and Gene Delivery
Product Description
The Lonza 4D Nucleofector is a modular electroporation system designed for high-efficiency, non-viral cell transfection and gene delivery across difficult and sensitive cell types, widely applied in molecular biology, immunology, and cell therapy research. Utilizing Amaxa nucleofection technology, it ensures reproducible transfection results coupled with high post-transfection viability, essential for applications in stem cell studies, immune cell engineering, and gene editing workflows like CRISPR-Cas9 delivery.
Key features include:
- Support for DNA, RNA, mRNA, and CRISPR component transfection with an extensive library of pre-optimized, cell type–specific electroporation programs.
- Modular design with interchangeable units:
- X-Unit for small-scale cuvette-based transfections (1–16 reactions at 20 µL or 1 reaction at 100 µL volume)
- Y-Unit for adherent cell electroporation
- LV-Unit for large-volume, continuous flow transfections scaling from 1 mL up to 20 mL, accommodating up to 10⁹ cells, supporting bioprocessing and biomanufacturing workflows.
- Maximum electrical parameters of 1500 V and 100 A using aluminum-free conductive polymer electrodes to optimize cell viability and transfection efficiency.
- Throughput and volume flexibility tailored for both discovery-phase experiments and scale-up in molecular diagnostics and biotechnology research.
This electroporation platform is critical in biomedical engineering, synthetic biology, and molecular cloning workflows, providing precise control over gene transfer essential for stem cell engineering, immunotherapy development, and biopharmaceutical production pipelines. Its proven capacity to transfect primary immune cells, including PBMCs, T cells, and CD34+ stem cells, enhances reproducibility and reduces experimental variability.
The compact system (approx. 33 lbs) operates on standard 100–240V power supply and integrates seamlessly into research labs focusing on live-cell imaging, fluorescence analysis, and advanced genetic manipulation techniques. This versatility, combined with robust performance, makes the Lonza 4D Nucleofector a widely adopted electroporation system for cutting-edge life science research and biotechnology applications.
Item Stats
Product details
| Specification | Core + X-Unit (Small Scale) |
| Throughput | 1–16 reactions (20 µL); 1 reaction (100 µL) |
| Volume Range | 20 µL (Strips) / 100 µL (Cuvettes) |
| Max Cell Density | 2 x 10⁴ to 2 x 10⁷ cells |
| Max Voltage / Current | 1500 V / 100 A |
| Electrode Material | Conductive Polymer (Aluminum-Free) |
| DNA/RNA Range | 0.2 – 5 μg (Format dependent) |
| System Weight | Approx. 33 lbs (Total System) |
| Power Requirements | 100–240V, 50-60Hz |
| Dimensions | 11 x 10 x 17 inches (Stacked) |
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