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ProteinSimple Wes Automated Capillary Western Blot System for Protein Separation and Immunodetection
Product Description
The ProteinSimple Wes Western Blot System is an automated, capillary-based platform designed for precise protein separation and immunodetection, widely adopted in molecular diagnostics and biotechnology research. It supports high-throughput analysis, processing up to 24 samples per run with a total assay time of approximately 3 hours, optimizing workflows in bioprocessing and synthetic biology.
Key technical features include:
- Capillary electrophoresis using 5 cm, 100 µm capillaries with 400 nL volume capacity
- Protein size range capability from 2 to 440 kDa, suitable for a broad spectrum of protein targets
- Minimal sample volume requirement of 3 µL per capillary to reduce reagent use and assay variation
- Dynamic detection range spanning 3-4 logs for immunoassays and 2-3 logs for total protein, supporting quantitative molecular diagnostics
- Sensitivity down to low picogram levels for immunodetection, essential for biomarker validation and biopharmaceutical research
- Integration with Compass software for advanced assay analysis, 21 CFR Part 11 compliance, digital capillary imaging, and robust data management
This system automates all critical steps: sample loading, size-based separation, immunoprobing, washing, detection, and analysis, replacing labor-intensive traditional Western blotting. Its high reproducibility (sizing CV <10%, intra-assay CV <15%) and advanced HDR detection technology ensure reliable results critical in biomanufacturing pipelines and live-cell protein expression studies.
The ProteinSimple Wes is distinct from multiplex ELISA instruments like the ProteinSimple Ella, focusing on capillary-based Western blotting rather than immunoassay multiplexing. It is an essential laboratory instrument for researchers in cell imaging, neuroscience, and biotechnology, streamlining workflows in gene editing and molecular cloning projects with enhanced accuracy and throughput.
Item Stats
Product details
| Proteins size range | 2-440 kDa |
| Minimum sample size | 3uL per capillary |
| Capillary | 5 cm, 100 µm, 400 nL |
| Maximum Number of Samples | 25 (75 samples per day) |
| Sizing CV | <10% |
| Intra-assay CV | <15% |
| Resolution (± percent difference in MW) | ± 15-20% for MW <20 kDA ± 10% for MW >20 kDa |
| Dynamic Range | 2-3 logs (Total Protein Specification)3-4 logs (Immunoassay Specification) |
| Sensitivity | ng(Total Protein Specification)Low pg (Immunoassay Specification) |
| Process Time | 3 hours |
| Dimensions | 14x21x13” |
| Weight | 50lbs |
| Power | 100-240vac, 50/80Hz, 510W |
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