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Agilent 1260 Infinity I HPLC System with VWD and FLD Detectors
Product Description
The Agilent 1260 Infinity I HPLC System is a versatile high-performance liquid chromatography instrument designed for method development and high-throughput applications. Key features include:
- Binary Pump: Integrated dual pumps delivering 0.001 to 5 mL/min flow rates, supporting up to 600 bar pressure for high-resolution gradients.
- Variable Wavelength Detector (VWD): Double-beam photometer with a deuterium lamp, wavelength range 190 to 600 nm, dual-wavelength mode, and time-programmable switching for enhanced sensitivity.
- Fluorescence Detector (FLD): Equipped with a high-intensity flash lamp, multi-wavelength spectral scanning from 200 to 1200 nm (excitation and emission), simultaneous multi-signal detection, and optional off-line cuvette measurements.
- Autosampler: Flow-through needle design for minimal carryover and high-speed injections; variable volume capabilities.
- Column Compartment and ALS Thermostat: Temperature control from 4°C to 40°C with high stability via Peltier elements to ensure reproducible retention times.
- Software: Included Agilent ChemStation Workstation Software provides comprehensive control for data acquisition, analysis, and instrument automation.
- Additional Features: Four-solvent selection for both isocratic and gradient elution; degassing via high-performance degasser with four vacuum chambers.
The system undergoes extensive diagnostic tests including detector filter and lamp intensity tests, pump pressure/leak tests, thermostat performance, autosampler function, and a standard 4-peak isocratic test to ensure compliance with factory specifications before shipment.
Item Stats
Product details
| VWD Detector | Specification |
| Detection Type | Double-beam photometer |
| Light Source | Deuterium lamp |
| Wavelength Range | 190 - 600 nm |
| Short Term Noise (ASTM) Single and Multi-Wavelength | < ± 0.5 × 10^-5 AU at 254 nm |
| Drift | 3 × 10^-4 AU/hr at 254 nm |
| Linearity | > 2.0 AU (5%) upper limit |
| Band Width | 6.5 nm typical |
| Maximum Data Rate | 55 Hz |
| Signal Data Rate | Up to 80 Hz |
| Spectra Data Rate | Up to 80 Hz |
| Flow Cell | Standard: 14 µL volume, 10 mm cell path length and 40 bar (580 psi) pressure maximum |
| Control and Data Evaluation | Agilent ChemStation for LC |
| FLD Detector | Specification |
| Detection Type | Multi-signal fluorescence detector with rapid on-line scanning capabilities and spectral data analysis |
| Performance Specifications | Single wavelength operation: • RAMAN (H2O) > 500 (noise reference measured at signal) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell • RAMAN (H2O) > 3000 (noise reference measured at dark value) Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell Dual wavelength operation: RAMAN (H2O) > 300 Ex 350 nm, Em 397 nm and Ex 350 nm, Em 450 nm, standard flow cell. |
| Light source | Xenon Flash Lamp, normal mode 20 W, economy mode 5 W, lifetime 4000 h |
| Pulse frequency | 296 Hz for single signal mode 74 Hz for economy mode |
| Excitation Monochromator | Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 300 nm |
| Emission Monochromator | Range: settable 200 nm - 1200 nm and zero-order Bandwidth: 20 nm (fixed) Monochromator: concave holographic grating, F/1.6, blaze: 400 nm |
| Reference System: | in-line excitation measurement |
| Timetable programing | up to 4 signal wavelengths, response time, PMT Gain, baseline behavior (append, free, zero), spectral parameters |
| Spectrum acquisition: | Excitation or Emission spectra Scan speed: 28 ms per datapoint (e.g. 0.6 s/spectrum 200 – 400 nm, 10 nm step) Step size: 1 – 20 nm Spectra storage: All |
| Wavelength characteristic | Repeatability+/- 0.2 nm Accuracy+/- 3 nm setting |
| Flow cells | Standard: 8 µl volume and 20 bar (2 MPa) pressure maximum, quartz Optional: Fluorescence cuvette for offline spectroscopic measurements with 1 ml syringe, 8 µl volume, quartz |
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Northeastern UniversityFrom €38,645.77*ex. VAT
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