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Invitrogen Attune NxT Acoustic Focusing Cytometer 2 with 3 Lasers (B3-R3-V6)
Product Description
- Acoustic focusing technology ensures precise cell alignment for consistent results
- High sensitivity detection suited for complex multiparametric flow cytometry
- Rapid sample processing optimizes throughput and reproducibility
- Configured with 3 lasers (B3-R3-V6) for flexible excitation options
- User-friendly software enhances instrument control and data analysis
- Applicable for both academic and clinical research requiring robust cell analysis
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Product details
| Description | The Invitrogen Attune NxT AFC2 is a benchtop flow cytometer utilizing acoustic focusing to improve sample flow and sensitivity. It features 3 lasers for multiparametric fluorescence detection and accommodates a variety of experimental protocols. The instrument supports modern software versions and includes essential accessories for operation and maintenance. |
| Technical Data | Model: Attune NxT AFC2 Serial Number: 2AFC239780622 Manufacturing Year: 2022 Lasers: Count: 3 Wavelengths: 405 nm (violet), 488 nm (blue), 638 nm (red) Warranty: 90-Days |
| Model | Attune NxT AFC2 |
| Serial Number | 2AFC239780622 |
| Manufacturing Year | 2022 |
| Lasers | Count: 3 Wavelengths: 405 nm (violet), 488 nm (blue), 638 nm (red) |
| Count | 3 |
| Wavelengths | 405 nm (violet), 488 nm (blue), 638 nm (red) |
| Warranty | 90-Days |
| Accessories | (Optional) CytKick/NxT Autosampler, (Optional) Containers, Laptop / (Desktop + Monitor) |
| , BSO 50mW, Max Diode power 100mW | , BSO 100mW, Max Diode power 140mW |
| , BSO 50mW, Max Diode power 100mW | , BSO 100mW, Max Diode power 140mW |
| , BSO 50mW, Max Diode power 100mW | , BSO 100mW, Max Diode power 140mW |
| Wavelength | Diode power |
| Laser excitation | Optimized excitation for minimized stray laser-line noise and losses to reflection |
| Laser profile | 10 x 50 ÎĽm flat-top laser providing robust alignment |
| Emission filters | 12 color channels with wavelength-tuned photomultiplier tubes (PMTs); user-changeable, keyed filters |
| Laser separation | 150 ÎĽm |
| Optical alignment | Fixed alignment with pre-aligned welded fiber; no user maintenance required |
| Onboard thermoelectric cooler | No warm-up delay; fiber isn’t affected by “on/off” |
| Simmer mode | Instant “on/off” reduces usage and/or aging by 10x; only turns on when acquiring samples; reports hours of usage |
| Flat-top laser specified at the flow cell | Coefficient of variation (CV) <3% over the width of the flat-top laser |
| Upgradable | Convenient field changes |
| Fluidics | Flow cell: Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm Sample analysis volume: 20 μL–4 mL Custom sample flow rates: 12.5–1,000 μL/min Sample delivery: Positive-displacement syringe pump for volumetric analysis Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm Fluid-level sensing: Active Standard fluid reservoirs: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank Fluid storage: All fluids stored within instrument Extended fluidics option: Configuration for 10 L fluid Nominal fluid consumption: 1.8 L/day Automated maintenance cycles: ≤15 min start-up and shutdown—deep clean, sanitize, and debubble modes |
| Flow cell | Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 ÎĽm |
| Sample analysis volume | 20 μL–4 mL |
| Custom sample flow rates | 12.5–1,000 μL/min |
| Sample delivery | Positive-displacement syringe pump for volumetric analysis |
| Sample tubes | Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm |
| Fluid-level sensing | Active |
| Standard fluid reservoirs | 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank |
| Fluid storage | All fluids stored within instrument |
| Extended fluidics option | Configuration for 10 L fluid |
| Nominal fluid consumption | 1.8 L/day |
| Automated maintenance cycles | ≤15 min start-up and shutdown—deep clean, sanitize, and debubble modes |
| Performance | Fluorescence detection Fluorescence sensitivity: ≤80 molecules of equivalent soluble fluorochrome (MESF) for FITC, ≤30 MESF for PE, ≤70 MESF for APC Fluorescence resolution: CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN) Data acquisition rate: Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics Maximum electronic speed: 65,000 events/sec with all parameters Carryover: Single-tube format: <1% Forward and side scatter sensitivity: Able to discriminate platelets from noise Forward and side scatter resolution: Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood Forward scatter: Photodiode detector with 488/10 nm bandpass filter Side scatter: PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter Fluorescence detectors: 14 individual detectors Electronic pulse: Measured area, height and width pulse for all detectors Violet side scatter resolution: Can be configured for violet side scatter to better resolve particles from noise Minimum particle size: 0.2 μm on side scatter using submicron bead calibration kit from Bangs Laboratories—0.1 μm on side scatter under following conditions: Using an Attune NxT Flow Cytometer with standard 0.5 mm blocking configuration, an Invitrogen Attune NxT 488/10 Filter (Cat. No. 100083194), and Attune Focusing Fluid (Cat. No. 4488621, 4449791, or A24904) that has been passed through a 0.025 µm filter |
| Fluorescence sensitivity | ≤80 molecules of equivalent soluble fluorochrome (MESF) for FITC, ≤30 MESF for PE, ≤70 MESF for APC |
| Fluorescence resolution | CV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN) |
| Data acquisition rate | Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statistics |
| Maximum electronic speed | 65,000 events/sec with all parameters |
| Carryover | Single-tube format: <1% |
| Forward and side scatter sensitivity | Able to discriminate platelets from noise |
| Forward and side scatter resolution | Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood |
| Forward scatter | Photodiode detector with 488/10 nm bandpass filter |
| Side scatter | PMT with default 488/10 nm bandpass filter; optional 405/10 nm bandpass filter |
| Fluorescence detectors | 14 individual detectors |
| Electronic pulse | Measured area, height and width pulse for all detectors |
| Violet side scatter resolution | Can be configured for violet side scatter to better resolve particles from noise |
| Minimum particle size | 0.2 μm on side scatter using submicron bead calibration kit from Bangs Laboratories—0.1 μm on side scatter under following conditions: Using an Attune NxT Flow Cytometer with standard 0.5 mm blocking configuration, an Invitrogen Attune NxT 488/10 Filter (Cat. No. 100083194), and Attune Focusing Fluid (Cat. No. 4488621, 4449791, or A24904) that has been passed through a 0.025 µm filter |
| Software Software requirements | Invitrogen Attune Cytometric Software Compensation: Full matrix—automated and manual modes, on-plot compensation tools for fine adjustment; use of tubes and wells Flow rate: Precise flow rate control via software; no hardware adjustments Live streaming: Live update of statistics during acquisition of events up to 35,000 events/sec Overlays: Comparative analysis between samples; 3D view Sample recovery: System able to return unused samples Concentration: Direct concentration measurement without use of counting beads Software layout: Fully customizable for each user account Bubble detection technology: Stops automated run to preserve sample integrity Maximum single-event file: 20 million with option to append Heat map: Set up for definition of plate layout; screening view for analysis for tubes and plates Threshold: Up to 4 individual thresholds with user option to apply Boolean logic Gating: Hierarchical gating with the ability to derive gates Voltage: User adjustable Window extensions: User adjustable Area scaling factor (ASF): User adjustable Acquisition settings: Documented in FCS files and maintained upon import Templates: Create from existing experiments—instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously Tube-to-plate conversion: One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes Graphics resolution: Publication-quality images; support for TIF, PNG, BMP, JPG, GIF, and EMF; quickly copy and paste plots to any external application (e.g., Microsoft™ PowerPoint™ software) User account administration: Administrative creation of individual user accounts with designated roles, advanced setting permissions, management of individual accounts, user time tracking, and sample count |
| Software requirements | Invitrogen Attune Cytometric Software |
| Compensation | Full matrix—automated and manual modes, on-plot compensation tools for fine adjustment; use of tubes and wells |
| Flow rate | Precise flow rate control via software; no hardware adjustments |
| Live streaming | Live update of statistics during acquisition of events up to 35,000 events/sec |
| Overlays | Comparative analysis between samples; 3D view |
| Sample recovery | System able to return unused samples |
| Concentration | Direct concentration measurement without use of counting beads |
| Software layout | Fully customizable for each user account |
| Bubble detection technology | Stops automated run to preserve sample integrity |
| Maximum single-event file | 20 million with option to append |
| Heat map | Set up for definition of plate layout; screening view for analysis for tubes and plates |
| Threshold | Up to 4 individual thresholds with user option to apply Boolean logic |
| Gating | Hierarchical gating with the ability to derive gates |
| Voltage | User adjustable |
| Window extensions | User adjustable |
| Area scaling factor (ASF) | User adjustable |
| Acquisition settings | Documented in FCS files and maintained upon import |
| Templates | Create from existing experiments—instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously |
| Tube-to-plate conversion | One-click transition from tubes to plates and vice versa; no disassembly, no additional QC, no reboot required for conversion between plates and tubes |
| Graphics resolution | Publication-quality images; support for TIF, PNG, BMP, JPG, GIF, and EMF; quickly copy and paste plots to any external application (e.g., Microsoft™ PowerPoint™ software) |
| User account administration | Administrative creation of individual user accounts with designated roles, advanced setting permissions, management of individual accounts, user time tracking, and sample count |
| Quality and regulatory Instrument tracking | Automated daily baseline and performance test with Levey-Jennings plots Warranty: 90-Days Production verification testing: Each instrument is tested and verified for assembly integrity and performance to specifications Quality management system: Manufacturing standards comply with the requirements of ISO 13485:2003 Robust installation specifications: Units installed by engineer; preplanning checklist, delivery, and installation; and performance validation compliance with standardized procedure Regulatory status: For Research Use Only |
| Instrument tracking | Automated daily baseline and performance test with Levey-Jennings plots |
| Warranty | 90-Days |
| Production verification testing | Each instrument is tested and verified for assembly integrity and performance to specifications |
| Quality management system | Manufacturing standards comply with the requirements of ISO 13485:2003 |
| Robust installation specifications | Units installed by engineer; preplanning checklist, delivery, and installation; and performance validation compliance with standardized procedure |
| Regulatory status | For Research Use Only |
| Installation requirements | Electrical requirements: 100–240 VAC, 50/60 Hz, <150 W. Thermo Fisher Scientific certifies that the Attune Flow Cytometers conform to relevant directives to bear the CE mark. The instrument also conforms to the UL and CAN/CSA general requirements (61010.1). The Attune Flow Cytometers are Class I laser products per Center for Devices and Radiological Health (CDRH) regulations and EN/IEC 60825. Heat dissipation: <150 W Temperature operating ranges: 15–30°C (59–86°F) Operating humidity: 10–90%, noncondensing Audible noise: <65 dBA at 1.0 m Instrument size: (H x W x D): ~40 x 58 x 43 cm (16 x 23 x 17 in.), including fluid bottles Weight: ~29 kg (64 lb) |
| Electrical requirements | 100–240 VAC, 50/60 Hz, <150 W. Thermo Fisher Scientific certifies that the Attune Flow Cytometers conform to relevant directives to bear the CE mark. The instrument also conforms to the UL and CAN/CSA general requirements (61010.1). The Attune Flow Cytometers are Class I laser products per Center for Devices and Radiological Health (CDRH) regulations and EN/IEC 60825. |
| Heat dissipation | <150 W |
| Temperature operating ranges | 15–30°C (59–86°F) |
| Operating humidity | 10–90%, noncondensing |
| Audible noise | <65 dBA at 1.0 m |
| Instrument size | (H x W x D): ~40 x 58 x 43 cm (16 x 23 x 17 in.), including fluid bottles |
| Weight | ~29 kg (64 lb) |
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