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Agilent 5400 Fragment Analyzer, Automation-Ready

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Product Description

The Agilent 5400 Fragment Analyzer is an automation-ready capillary electrophoresis analyzer for automated DNA and RNA fragment analysis, sizing, quantification, and quality assessment. It uses multiplexed capillary electrophoresis with fluorescence-based detection, a high-sensitivity CCD detector, and LED excitation.

Applications
  • DNA fragment sizing and quantification
  • RNA integrity and quality assessment
  • Next-generation sequencing library quality control
  • Genomic DNA integrity analysis
  • Total RNA and small RNA evaluation
  • PCR product verification
  • mRNA vaccine characterization and quality control
  • Molecular biology, biotechnology, genomics, and biopharmaceutical research workflows
Technical Specifications
  • Analysis method: Automated multiplexed capillary electrophoresis
  • Detection: Fluorescence-based detection with high-sensitivity CCD detector and LED excitation
  • Capillary array compatibility: 96-capillary arrays
  • Sample capacity: 1 × 96-well PCR sample plate
  • Samples per run: Up to 96 samples
  • Recommended sample input: 2 µL
  • Minimum volume per well for injection: 20 µL
  • Resolution: Up to 3 bp
  • Separation resolution: ≤5%
  • Sizing accuracy: ±10%
  • Power requirements: 100–240 VAC, 50/60 Hz
  • Dimensions (H × W × D): 86.4 × 101.6 × 61 cm (34 × 40 × 24 in)
  • Weight: 39 kg (86 lb)

The system supports full-skirt 96-well PCR plates for sample and marker preparation, plus 96-well deep-well plates for buffer and waste reservoirs. Its automation-ready design can support walk-away operation when integrated with compatible automation arms or automated liquid handlers.

Included Equipment
  • Agilent 5400 Fragment Analyzer
  • Three marker/sample PCR microplate holders for semi-skirted or non-skirted plates
  • Two deep-well microplate holders
  • One 50 mL bottle
  • Two 250 mL bottles
  • One 500 mL waste bottle
  • Computer with Fragment Analyzer controller software
  • Communication and power cables

Capillary arrays and other consumables are not included. The listed consumables were used for demonstration purposes.

The pre-owned instrument was tested for power-supply stability, software communication, initialization, homing, pump operation, plate movement, fluid priming, pressurization, and high-voltage operation.


Item Stats

Status
For Sale
State
used
Brand
Model
5400
Location
United States

Product details

StockIn Stock
BrandAgilent
Product CodeSM101495-INFINITY2
ConditionPre-Owned

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