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This model was found at
1295 locations
The model is used in
55 countries
Usage per year (up to 2020)
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About the Jeol JEM 1400

The model Jeol JEM 1400 was found in 1295 unique locations in 55 countries where it was mentioned from 2008 until recentlyIt is used by scientists in various research fields such as General Materials Science, Molecular Biology, General Chemistry, General Biochemistry, Genetics and Molecular Biology, and General Medicine. The model is also used in Cell Biology, General Physics and Astronomy, General Chemical Engineering, Biochemistry, Physical and Theoretical Chemistry, Pharmaceutical Science, Microbiology, Genetics, Organic Chemistry, Molecular Medicine, Cancer Research, Catalysis, Immunology, Biotechnology, Cellular and Molecular Neuroscience, Microbiology (medical), Virology, Drug Discovery, Analytical Chemistry, Bioengineering, Oncology, Spectroscopy, Inorganic Chemistry, Condensed Matter Physics, and Biomedical Engineering.
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Research that uses the Jeol JEM 1400

Kouji Adachi, Naga Oshima, Sho Ohata, Atsushi Yoshida, Nobuhiro Moteki, Makoto Koike, 2020
Abstract. Aerosol particles were collected at various altitudes in the Arctic during the Polar Airborne Measurements and Arctic Regional Climate Model Simulation Project (PAMARCMiP 2018) conducted in the early spring of 2018. The composition, size, number fraction, and mixing state of individual aerosol particles were analyzed using transmission electron microscopy (TEM), and their sources and transport were evaluated by numerical model simulations. We found that sulfate, sea-salt, mineral-dust, K-bearing, and carbonaceous particles were the major aerosol constituents and were internally mixed. The number fraction of mineral-dust and sea-salt particles decreased with increasing altitude. The K-bearing particles increased within a biomass burning (BB) plume at altitudes > 3900 m, which originated from Siberia. Chlorine in sea-salt particles was replaced with sulfate at high altitudes. These results suggest that the sources, transport, and aging of Arctic aerosols largely vary depending on the altitude and airmass history. We also provide the occurrences of solid-particle inclusions (soot, fly-ash, and Fe-aggregate particles), some of which are light-absorbing and potential ice-nucleating particles. Our TEM measurements revealed, for the first time, the detailed mixing state of individual particles at various altitudes in the Arctic. This information facilitates the accurate evaluation of the aerosol influences on Arctic haze, radiation balance, cloud formation, and snow/ice albedo when deposited.
Joshua N. Hamm, Susanne Erdmann, Emiley A. Eloe-Fadrosh, Allegra Angeloni, Ling Zhong, Christopher Brownlee, Timothy J. Williams, Kirston Barton, Shaun Carswell, Martin A. Smith, Sarah Brazendale, Alyce M. Hancock, Michelle A. Allen, Mark J. Raftery, Ricardo Cavicchioli, Proceedings of the National Academy of Sciences, 116, 14661-14670 (29), 2019
Significance We demonstrate that Candidatus Nanohaloarchaeum antarcticus requires Halorubrum lacusprofundi for growth, illustrating that Nanohaloarchaeota require a host rather than being free living as previously proposed. Developing the means of cultivating Nanohaloarchaeota in the laboratory provides the capacity to advance understanding of how archaea interact and the factors that control their symbiotic relationship (e.g. mutualism, commensalism, antagonism). Our findings amplify the view that Antarctic lakes are a treasure trove for the discovery of microbes with previously unknown properties.
Vanisri Raviraj, Binh T.T. Pham, Nguyen T.H. Pham, Byung J. Kim, Lai F. Kok, Nicole Painter, Naomi C. Delic, Stephen K. Jones, Brian S. Hawkett, J. Guy Lyons, 2020
Abstract Background: The skin is both a target and a potential conduit for the delivery of drugs, but its cornified cell layer resists penetration by most molecules. This study investigated the potential of superparamagnetic iron oxide nanoparticles to facilitate the transdermal delivery of anti-cancer agents. Results: Chemotherapeutic cancer drugs were applied with or without nanoparticles to the skin of hairless mice, and their ability to penetrate the skin was assessed using fluorescence microscopy and tumor growth. Nanoparticles enhanced the penetration of the skin by doxorubicin and 5-fluorouracil as determined by fluorescence microscopy and growth retardation of experimental melanoma in immunocompetent, syngeneic mice. This drug enhancement did not require conjugation or encapsulation of the drugs by the nanoparticles – simple co-administration sufficed. Nanoparticles applied topically to melanomas increased the cytotoxicity and immune cell infiltration induced by co-administered 5-fluorouracil, and also reduced vascularization of the tumors independently of 5-fluorouracil. Conclusion: Correctly formulated superparamagnetic iron oxide nanoparticles can facilitate the chemotherapeutic effectiveness of cytotoxic drugs on skin tumors by both increasing their transdermal penetration and ameliorating host-tumor interactions. This enhancement of skin penetration occurs without the need for conjugation or encapsulation of the co-administered drugs and so will likely be applicable to other drugs, also.
Paul Velander, Ling Wu, Sherry B. Hildreth, Nancy J. Vogelaar, Biswarup Mukhopadhyay, Shijun Zhang, Richard F. Helm, Bin Xu, 2020
Abstract Background: A range of neurodegenerative and related aging diseases, such as Alzheimer’s disease, Parkinson’s disease, and type 2 diabetes, are linked to toxic protein aggregation. Yet the mechanisms of protein aggregation inhibition by small molecule inhibitors remain poorly understood, in part because most protein targets of aggregation assembly are partially unfolded or intrinsically disordered, which hinders detailed structural characterization of protein-inhibitor complexes and structural-based mechanistic elucidation. Methods: Herein we employed a small molecule screening approach to identify inhibitors against three prototype amyloidogenic proteins in neurodegeneration and related proteinopathies: amylin, Ab and tau. We further systematically investigated selected class of inhibitors under aerobic and anaerobic conditions to uncover a key determinant of the inhibitory activities.Results: One remarkable class of inhibitors identified from all three parallel screenings against different amyloidogenic proteins was catechol-containing compounds and redox-related quinones/anthraquinones. Further mechanistic studies determined that the redox state of the broad class of catechol-containing compounds is a key determinant of the amyloid inhibitor activities. Conclusion: Our small molecule library screening platform was able to identify a broad class of amyloid inhibitors. Redox was found to be a key factor not only regulating the inhibitory activities but also involving the mechanism of inhibition. The molecular insights we gained not only explain why a large number of catechol-containing natural compounds, often enriched in healthy diet, have anti-neurodegeneration and anti-aging activities, but also could guide the rational design of therapeutic or nutraceutical strategies to target a broad range of neurodegenerative and related aging diseases.
Kangyu Zheng, Zeju Huang, Jiaying Huang, Xiangmei Liu, JUNFENG BAN, Xin Huang, Haosen Luo, Zhicong Chen, Qingchun Xie, Yanzhong Chen, Zhufen Lu, 2020
Abstract Background: The aim of this work was to develop a novel and feasible modification strategy by utilizing the supramolecular effect of 2-hydroxypropyl-beta-cyclodexrin (2-HP-β-CD) for enhancing the biological transport efficiency of paclitaxel (PTX)-loaded poly(lactide-co-glycolide)(PLGA) nanoparticles.Methods: PTX-loaded 2-HP-β-CD-modified PLGA nanoparticles (2-HP-β-CD/PLGA NPs) were prepared using the modified emulsion method. Nano-characteristics, drug release behavior, in vitro cytotoxicity, cellular uptake profiles and in vivo bio-behavior of the nanoparticles were then characterized. Results: Compared with the plain PLGA NPs, 2-HP-β-CD/PLGA NPs exhibited smaller particle sizes (151.03±1.36 nm), increased entrapment efficiency (~49.12% increase) and sustained drug release. When added to A549 human lung cancer cells, compared with PLGA NPs, 2-HP-β-CD/PLGA NPs exhibited higher cytotoxicity in MTT assays and improved cellular uptake efficiency. Pharmacokinetic analysis showed that the AUC value of 2-HP-β-CD/PLGA NPs was 2.4-fold higher than commercial Taxol® and 1.7-fold higher than plain PLGA NPs. In biodistribution assays, 2-HP-β-CD/PLGA NPs exhibited excellent stability in the circulation.Conclusions: The results of this study suggest that formulation contains 2-HP-β-CD can prolong PTX release, enhance drug transpot efficiency and serve as a potential tumor targeting system for PTX.
Sumera Sabir, Muhammad Asif Zahoor, Muhammad waseem, Muhammad Hussnain Siddique, Muhammad Shafique, Muhammad Imran, Sumreen Hayat, Imran Riaz Malik, Saima Muzammil, 2020
Abstract The authors have withdrawn this preprint due to erroneous posting.
Masahiko Kashin, Yasumasa Kakei, Shun Teraoka, Takumi Hasegawa, Akinobu Yamaguchi, Takao Fukuoka, Ryohei Sasaki, Masaya Akashi, BioMed Research International, 2020, 1-10, 2020
Cetuximab, an epidermal growth factor receptor inhibitor (EI), is currently the only targeted molecular therapy used in combination with radiotherapy for head and neck squamous cell carcinoma (HNSCC). Gold nanoparticles (AuNPs) are expected to enhance radiotherapy effects in cancers. To investigate whether AuNPs combined with AG1478, an EI, enhanced irradiation effects on HNSCC cells, we first examined AG1478 adsorption on AuNP surfaces, using surface-enhanced Raman scattering, which indicated an adsorption equilibrium of AG1478 to AuNPs. We then used transmission electron microscopy to find internalization rates of AuNP alone and AuNP+AG1478; we found that intracellular uptake of AuNP alone and AuNP+AG1478 did not significantly differ. We compared cell numbers, proliferation, apoptosis, and migration between control cells and those treated with or without 60 nm AuNP (1.0 nM), AG1478 (0.5 μM), and irradiation (4 Gy). We found that AuNP+AG1478 inhibited proliferation more than AG1478 alone; the combination of irradiation+AuNP+AG1478 significantly reduced total cell numbers compared with the combination of irradiation+AuNP; AuNP+AG1478 increased apoptotic reaction to irradiation; the combinations of AuNP+AG1478 and irradiation+AuNP induced more apoptosis than AG1478+irradiation. Whereas AuNP+AG1478 enhanced cytotoxicity in human HNSCC cells by inhibiting proliferation, irradiation+AuNP enhanced cytotoxicity by inducing apoptosis.
Botond Zsombor Pertics, Dalma Szénásy, Dániel Dunai, Yannick Born, Lars Fieseler, Tamás Kovács, György Schneider, BioMed Research International, 2020, 1-10, 2020
Methicillin-resistant Staphylococcus aureus (MRSA) can cause a wide range of infections from mild to life-threatening conditions. Its enhanced antibiotic resistance often leads to therapeutic failures and therefore alternative eradication methods must be considered. Potential candidates to control MRSA infections are bacteriophages and their lytic enzymes, lysins. In this study, we isolated a bacteriophage against a nosocomial MRSA strain belonging to the ST45 epidemiologic group. The phage belonging to Caudovirales, Siphoviridae, showed a narrow host range and stable lytic activity without the emergence of resistant MRSA clones. Phylogenetic analysis showed that the newly isolated Staphylococcus phage R4 belongs to the Triavirus genus in Siphoviridae family. Genetic analysis of the 45 kb sequence of R4 revealed 69 ORFs. No remnants of mobile genetic elements and traces of truncated genes were observed. We have localized the lysin (N-acetylmuramoyl-L-alanine amidase) gene of the new phage that was amplified, cloned, expressed, and purified. Its activity was verified by zymogram analysis. Our findings could potentially be used to develop specific anti-MRSA bacteriophage- and phage lysin-based therapeutic strategies against major clonal lineages and serotypes.
Chang Liu, Xiaoyu Xu, Junnian Zhou, Jiaqi Yan, Dongqing Wang, Hongbo Zhang, 2020
Abstract Metal-organic frameworks (MOFs) have proven to be a promising class of drug carriers due to their high porosity, crystalline properties with defined structure information, and their potential for further functionalization. However, to date, no extensive research has been conducted on MOF-based drug carriers with stimuli-responsive, dual-drug delivery, and tumor targeting functions. Here, we demonstrate the strategy of constructing a redox responsive and tumor-targeted MOF, as dual-drug carrier, by anchoring functional disulfide anhydride and folic acid (FA) molecules to the organic links of MOFs, respectively. The MOF composites show the controlled release of loaded 5-fluorouracil (5-FU) entrapped within UiO-66-NH2 nanostructures modified by dichloroacetic acid (DCA). Moreover, the MOF building block DCA acts as a synergistical drug to 5-FU in cancer cells inhibition. Through disulfide bonds, the gated MOF has redox-responsive drugs release. The confocal laser scanning microscopy further proved that conjugation of folic acid to the MOF surface can significantly enhance the targeting ability to cancer cells and the cancer cell uptake of FA-MOFs. The synthesis of redox-responsive dual-drug delivery MOF hybrids paves the way to assemble of other MOF hybrids that respond to other triggering factors such as light, temperature, pH, or biomarkers. The properties and functions of such materials are expected to influence the development of sensors, new catalysts, photonic devices, and drug delivery carriers.
Canh Minh Thang Nguyen, Vinh Tien Nguyen, Advances in Materials Science and Engineering, 2020, 1-9, 2020
We prepared silver nanoparticles (AgNP) embedded in SiO2 using a green polyol approach by conducting the synthesis at ambient temperature and pH. Glycerol solutions of SiO2 and silver nitrate were stirred overnight at room temperature. UV-vis spectra and TEM images of the reaction dispersion and XRD patterns of the centrifuged solid confirmed formation of AgNP (6 ± 2 nm) were embedded in SiO2. AAS showed that, about 50% of initial silver was deposited on SiO2. The presence of SiO2 enhanced the formation of AgNP and the stability of Ag/SiO2 in glycerol. The reason for these findings was probably the ultrasonic-probe dispersion of SiO2 in glycerol, which caused chemical interactions between glycerol and SiO2. Compared to bare AgNP, the AgNP/SiO2 demonstrated higher catalytic activity toward 4-nitrophenol reduction by NaBH4. The highest apparent rate constant was approximately 1.1 ∗ 10−4 s−1, comparable with Ag/SiO2 catalysts prepared using other methods. This study proposes a greener polyol method to synthesize SiO2-supported AgNP catalyst that does not require heating or regulating pH of the reaction mixture. This nanocomposite can be used in catalytic, antimicrobial, sensing, and other applications that are using AgNP/SiO2 synthesized by conventional methods.
Nguyen Lam Uyen Vo, Thi Thuy Van Nguyen, Tri Nguyen, Phung Anh Nguyen, Van Minh Nguyen, Ngoc Huy Nguyen, Van Linh Tran, Ngoc Anh Phan, Ky Phuong Ha Huynh, Journal of Nanomaterials, 2020, 1-13, 2020
In this study, CuO-ZnO composite was synthesized via the sol-gel method using oxalic acid to form the medium complex and its applications in antibacterial have been conducted with B. cereus, E. coli, S. aureus, Salmonella, and P. aeruginosa. Then, nanopowder of CuO-ZnO was coated on shoe insoles and their antibacterial effect with S. aureus was tested. The nanocomposite products were characterized by XRD, XPS, SEM, TEM, and UV-Vis. The results showed that the CuO-ZnO composite has the average particle size in a range of 20-50 nm, the point of zero charge of 7.8, and the bandgap of 1.7 eV. XPS result shows the composite structure with Cu2+ in the product. The minimum inhibitory concentration (MIC) of CuO-ZnO nanocomposite was 0.313 mg·mL-1 for S. aureus and Samonella, 0.625 mg·mL-1 for E. coli, and 5 mg·mL-1 for B. cereus and P. aeruginosa. The shoe insoles coated with 0.35 wt.% of CuO-ZnO nanocomposite also had high antibacterial activity against S. aureus, and this antibacterial nanocomposite was implanted durably on the surface of the shoe insoles.
Minh Thanh Vu, Ngoc Thuy Trang Le, Truc Le-Buu Pham, Ngoc Hoi Nguyen, Dai Hai Nguyen, Journal of Nanomaterials, 2020, 1-9, 2020
In the present work, a dual-drug-loaded soy lecithin liposomal system was developed by coencapsulation of Letrozole (LET) with Paclitaxel (PTX) to improve the efficacy in breast cancer therapy. Liposomes were synthesized by the thin film layer hydration. To sufficiently evaluate the characteristics of these liposomes, the particle size, zeta potential, morphology, drug encapsulation, in vitro drug release, and cytotoxicity were ascertained. Results showed promisingly anticancer potentials, as the following parameters indicated: nanosize diameter (around 193 nm) and negative surface charge. Data collected from the coloaded drug liposomes showed suitable encapsulation efficiency (50.56% for PTX and 31.13% for LET). Controlled and sustained releases were achieved up to 72 h for both the loaded drugs following the diffusion mechanism. In addition, the in vitro cytotoxicity study on the human breast cancer cell line (MCF-7) given the dual-drug-loaded liposome showed greater inhibition of cell growth than the single drug. Consequently, LET and PTX coloaded liposomes made from soy lecithin are expected to be an ingenious drug-delivery system for combination chemotherapy.
Shiyu Song, Shuyin Bao, Chenghong Zhang, Jinwei Zhang, Jiajun Lv, Xiaoyu Li, Maryam Chudhary, Xiang Ren, Li Kong, Oxidative Medicine and Cellular Longevity, 2021, 1-15, 2021
Diabetic retinopathy (DR) is a kind of severe retinal neurodegeneration. The advanced glycation end products (AGEs) affect autophagy, and mitochondrial function is involved in DR. Adenosine-activated protein kinase (AMPK) is an important metabolic sensor that can regulate energy homeostasis in cells. However, the effect of AMPK in DR is still not fully understood. In this study, we investigated the effect of AMPK on diabetes-induced photoreceptor cell degeneration. In vivo, a diabetic mouse model was established by streptozotocin (STZ) injection. Haematoxylin-eosin (HE) staining was used to observe retinal morphology and measure the thicknesses of different layers in the retina. Electroretinogram (ERG) was used to evaluate retinal function. In vitro, 661w cells were treated with AGEs with/without an AMPK agonist (metformin) or AMPK inhibitor (compound C). Flow cytometry and CCK-8 assays were used to analyse apoptosis. Mitochondrial membrane potential was analysed by JC-1. Western blotting and qRT-PCR were used to examine the expression of related proteins and genes, respectively. The wave amplitude and the thickness of the outer nuclear layer were decreased in diabetic mice. The expression of rhodopsin and opsin was also decreased in diabetic mice. In vitro, the percentage of apoptotic cells was increased, the expression of the apoptosis-related protein Bax was increased, and Bcl-2 was decreased after AGE treatment in 661w cells. The expression of the autophagy-related protein LC3 was decreased, and p62 was increased. The mitochondrial-related gene expression and membrane potential were decreased, and mitochondrial morphology was abnormal, as observed by TEM. However, AMPK stimulation ameliorated this effect. These results indicate that AMPK stimulation can delay diabetes-induced photoreceptor degeneration by regulating autophagy and mitochondrial function.
Qin Hu, Shu Zhang, Jun Zhu, Lina Yin, Suping Liu, Xiaowei Huang, Guihao Ke, BioMed Research International, 2021, 1-7, 2021
Endometrial cancer (EC) is a common gynecological malignancy worldwide whose therapy mainly depends on chemotherapy. In past years, an increasing number of studies indicate that hollow MnO2 could serve as a nanoplatform in the drug delivery system. The Brucea javanica oil emulsion (BJOE) has been illustrated to play a vital role in cancers. However, knowledge about the combined effect of H-MnO2-PEG/BJOE in endometrial cancer remains ambiguous up to now. In the present work, we prepared a drug-delivery vector H-MnO2-PEG by chemical synthesis and found that H-MnO2-PEG significantly inhibited cell proliferation in endometrial cancer cells. Moreover, the combination of H-MnO2-PEG/BJOE could repress cell proliferation more efficiently and promote cell apoptosis. Mechanistically, we found that BJOE exerted its role as a promoter of endometrial apoptosis by regulating relative protein expressions. In general, the present study demonstrates that H-MnO2-PEG functions as a critical vector in the tumor microenvironment of endometrial cancer and the significant effect of H-MnO2-PEG/BJOE on cancer cells, suggesting a new paradigm for the treatment of endometrial cancer.
Ngoc-Dung Huynh Luu, Le Hang Dang, Hoang Minh Bui, Trang Thuy Thi Nguyen, Bich Tram Nguyen, Le Son Hoang, Ngoc Quyen Tran, Journal of Nanomaterials, 2021, 1-12, 2021
Chromolaena odorata is a medicinal herb with prominent pharmacological properties. The therapeutic efficiency of Chromolaena odorata extracts and its ingredients have, however, been limited by various factors, including the lack of targeting capacity and poor bioavailability. To approach this drawback, ethyl acetate fraction extract of Chromolaena odorata- (EA.ChO-) encapsulated pluronic-based nanocarriers was disclosed herein. The most common pluronic triblock copolymer micelles (pluronic F127) was used for the nanosized formulation of Chromolaena odorata extract. The obtained results show that EA.ChO-encapsulated nanoparticles have a spherical morphology with a designed hydrodynamic size was about 183.7 nm and zeta potential -39.5 mV. The EA.ChO nanoparticles are stable in different aqueous solutions (water, PBS 2.8, and PBS 7.4). The lyophilized form of the EA.ChO nanoparticles exhibited excellent stability for long-term storage. Notably, the EA.ChO nanoparticles were 1.3-1.4 fold more effective in the growth of fibroblast than the free EA.ChO, verifying the potential of pluronic F127 nanoparticles to the increased function of EA.ChO in the proliferation of fibroblast cell. In addition, bleeding stopped within 55 ± 6  s which was 20 s faster than that of free EA.ChO and 38-44 s faster than that of negative control treatments. The EA.ChO nanoencapsulation processed a rapid blood clot formation compared to control, free EA.ChO, pluronic F127, and water, suggesting the excellent bioavailability of EA.ChO nanoencapsulation. The obtained results thus provided a promising prospect for raising the activity Chromolaena odorata extract in wound healing application.
Duy Khang Nguyen Vu, Dang Khoa Vo Nguyen, Journal of Nanomaterials, 2021, 1-14, 2021
In this work, silver nanoparticle- (AgNP-) embedded graphene oxide- (GO-) TiO2 nanotube (TNT) nanocomposite (labelled GAT) was successfully synthesized by gamma ray radiolysis. The influence of irradiation process, including one-step and two-step assistances and at different irradiation doses (5, 10, 15, 20, and 25 kGy), on the GAT’s physicochemical properties was achieved. Structure and properties of irradiated materials were analyzed by Fourier-transformed infrared (FT-IR), ultraviolet-visible absorption (UV-Vis), and Raman spectroscopies; X-ray diffraction (XRD); and scanning electron (SEM) and transmission electron (TEM) microscopies. In addition, selective scavengers of e-aq and ⋅OH radicals were used to investigate the radiolytic synthesis of GAT nanocomposite. It was revealed that gamma ray irradiation could strongly support the relation of the composite synthesis. Furthermore, the synthesized GAT nanocomposites showed a significant effect for Rhodamine B (RhB) photodecomposition after 60 minutes of natural sunlight exposure and evaluation by UV-Vis absorption spectroscopy. Briefly, the obtained results highlighted the potential of gamma irradiation as a “clean” and controllable way for synthesizing beneficial nanocomposite materials for wastewater purification and other environmental aspects.
Haiqiao Zhang, Yan Wu, Feng Yang, Huiling Dong, Yuqing Bian, Huanliang Jia, Xuqin Xie, Jilei Zhang, Frontiers in Bioengineering and Biotechnology, 9, 2021
Cellulose nanocrystal (CNC) has been applied in various fields due to its nano-structure, high aspect ratio, specific surface area and modulus, and abundance of hydroxy groups. In this work, CNC suspensions with different concentrations (0.4, 0.6, and 0.8%) were used as the adjuvant to improve the dispersion ability of multilayer graphene (MLG) in aqueous suspension, which is easy to be aggregated by van der Waals force between layers. In addition, N-methyl-2-pyrrolidone, ethanol, and ultrapure water were used as control groups. Zeta potential analysis and Fourier transform infrared spectroscopy showed that the stability of MLG/CNC has met the requirement, and the combination of CNC and MLG was stable in aqueous suspension. Results from transmission electron microscopy, Fourier transform infrared spectroscopy, and absorbance showed that MLG had a better dispersion performance in CNC suspensions, compared to the other solutions. Raman spectrum analysis showed that the mixtures of 1.0 wt% MLG with 0.4% CNC had the least defects and fewer layers of MLG. In addition, it is found that CNC suspension with 0.8% concentration showed the highest ability to disperse 1.0 wt% MLG with the most stable performance in suspension. Overall, this work proved the potential application of CNC as adjuvant in the field of graphene nanomaterials.
Manh B. Nguyen, Tuyen V. Nguyen, Giang H. Le, Trang T. T. Pham, Khu Le Van, Giang T.T. Pham, Tung Ngoc Nguyen, Quang Vinh Tran, Tuan A. Vu, Journal of Chemistry, 2021, 1-12, 2021
An atomic implantation method was used to modify diatomite with CuCl. The CuCl/diatomite samples were characterized by different techniques, including FTIR, XRD, BET, SEM-TEM, EDX, and CO-TPR. Characterization results revealed the formation of CuCl particles of 50–60 nm highly dispersed on diatomite surface. CO adsorption measurements showed that 2CuCl/diatomite exhibits the highest CO adsorption capacity among all CuCl-modified samples with diatomite. Its CO adsorption capacity of 2.96 mmol/g at 30°C is 10 times higher than that of unmodified diatomite (0.29 mmol/g). The CO adsorption on CuCl-modified diatomites was found to fit well with the Langmuir–Freundlich model.
Jin Cheng, Xiaoning Duan, Xin Fu, Yanfang Jiang, Peng Yang, Chenxi Cao, Qi Li, Jiying Zhang, Xiaoqing Hu, Xin Zhang, Yingfang Ao, Frontiers in Cell and Developmental Biology, 9, 2021
Osteoarthritis (OA) is a highly prevalent and debilitating joint disorder that characterized by progressive destruction of articular cartilage. There is no effective disease-modifying therapy for the condition due to limited understanding of the molecular mechanisms on cartilage maintenance and destruction. Receptor-interacting protein kinase 1 (RIP1)-mediated necroptosis plays a vital role in various diseases, but the involvement of RIP1 in OA pathogenesis remains largely unknown. Here we show that typical necrotic cell morphology is observed within human OA cartilage samples in situ, and that RIP1 is significantly upregulated in cartilage from both OA patients and experimental OA rat models. Intra-articular RIP1 overexpression is sufficient to induce structural and functional defects of cartilage in rats, highlighting the crucial role of RIP1 during OA onset and progression by mediating chondrocyte necroptosis and disrupting extracellular matrix (ECM) metabolism homeostasis. Inhibition of RIP1 activity by its inhibitor necrostatin-1 protects the rats from trauma-induced cartilage degradation as well as limb pain. More importantly, we identify bone morphogenetic protein 7 (BMP7) as a novel downstream target that mediates RIP1-induced chondrocyte necroptosis and OA manifestations, thereby representing a non-canonical regulation mode of necroptosis. Our study supports a model whereby the activation of RIP1-BMP7 functional axis promotes chondrocyte necroptosis and subsequent OA pathogenesis, thus providing a new therapeutic target for OA.
Yeoung-Hyun Park, Chul Son, You-Mi Seo, Yoon Seon Lee, Alix Har, Joo-Cheol Park, Frontiers in Cell and Developmental Biology, 9, 2021
Dentin, which composes most of the tooth structure, is formed by odontoblasts, long-lived post-mitotic cells maintained throughout the entire life of the tooth. In mature odontoblasts, however, cellular activity is significantly weakened. Therefore, it is important to augment the cellular activity of mature odontoblasts to regenerate physiological dentin; however, no molecule regulating the cellular activity of mature odontoblasts has yet been identified. Here, we suggest that copine-7 (CPNE7) can reactivate the lost functions of mature odontoblasts by inducing autophagy. CPNE7 was observed to elevate the expression of microtubule-associated protein light chain 3-II (LC3-II), an autophagy marker, and autophagosome formation in the pre-odontoblast and mature odontoblast stages of human dental pulp cells. CPNE7-induced autophagy upregulated DSP and DMP-1, odontoblast differentiation and mineralization markers, and augmented dentin formation in mature odontoblasts. Furthermore, CPNE7 also upregulated NESTIN and TAU, which are expressed in the physiological odontoblast process, and stimulated the elongation of the odontoblast process by inducing autophagy. Moreover, lipofuscin, which progressively accumulates in long-lived post-mitotic cells and hinders their proper functions, was observed to be removed in recombinant CPNE7-treated mature odontoblasts. Thus, CPNE7-induced autophagy reactivated the function of mature odontoblasts and promoted the formation of physiological dentin in vivo. On the other hand, the well-known autophagy inducer, rapamycin, promoted odontoblast differentiation in pre-odontoblasts but did not properly reactivate the function of mature odontoblasts. These findings provide evidence that CPNE7 functionally reactivates mature odontoblasts and introduce its potential for dentinal loss-targeted clinical applications.
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