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BD FACSymphony X-50 Five-Laser Multiparameter Flow Cytometer with HTS Sampler & Supply System
Product Description
The BD FACSymphony X-50 Flow Cytometer is a high-performance multiparameter cell analyzer equipped with five lasers and capable of detecting up to 28 fluorescent colors simultaneously through 30 configurable detector channels. This system integrates a High-Throughput Sampler (HTS) compatible with 96- and 384-well plates, enabling automated, plate-based sample acquisition essential for bioprocessing and molecular diagnostics applications.
Optical Configuration:
- Lasers: UV 355 nm (65 mW), Violet 405 nm (200 mW), Blue 488 nm (400 mW), Yellow-Green 532 nm (100 mW), Red 637 nm (100 mW)
- 28 fluorescence parameters with capacity up to 50 parameters upon upgrade
- Forward scatter (FSC) and side scatter (SSC) detectors optimized for particle sizing and granularity
- Precise filter sets tailored to each laser for optimal spectral resolution
Performance Specifications:
- Fluorescence resolution: CV <3.0% for PI-stained cells (Full G0/G1 peak)
- Acquisition throughput: up to 40,000 events/second with bead standards
- Fluidics system supports adjustable sample flow rates: LO (12 µL/min), MED (35 µL/min), HI (60 µL/min)
- Low sample carryover (<0.5% in HTS mode) ensures high data integrity for high-throughput experiments
Fluidics and Sample Handling:
- Robust FACSFlow Supply System maintains stable fluidic pressure and flow
- Multiple flow modes (RUN, STANDBY, PRIME) for flexible operation
- High sensitivity and resolution allow discrimination of rare cell populations, critical for advanced immunophenotyping and single-cell analysis
Software and Workflow Integration:
Includes a 1-year subscription to De Novo FCS Express 7 Analysis Software (RUO version) featuring:
- Compatibility with diverse flow cytometry data formats (FCS, BD FACSDiva, Beckman Coulter, Cytobank, etc.)
- Advanced gating, kinetic analysis, and statistical tools essential for molecular biology and gene editing workflows
- Real-time data visualization and customizable automated reporting
- Cloud-enabled collaboration for team-based biotechnological research
Applications:
- Complex multicolor immunophenotyping for clinical diagnostics and biotechnology research
- Rare cell detection and characterization
- Intracellular cytokine signaling and cell cycle studies
- High-throughput screening essential in synthetic biology and biopharmaceutical production pipelines
Quality Assurance and Service:
The instrument undergoes comprehensive service testing, including:
- Optical alignment and laser power verification at mW precision
- Fluidics system decontamination and pressure profiling
- Calibration with standardized bead sets ensuring reproducible coefficient of variation (CV) values
- Validation of detector sensitivity and noise levels to maintain signal fidelity
- Comes fully warranted with options for on-site or remote installation and training
Keywords:
multiparameter flow cytometer, cell analyzer flow cytometer, five-laser flow cytometer, high-throughput sampler, immunophenotyping instrument, molecular diagnostics tool, bioprocessing analytics, synthetic biology research, rare cell detection, fluorescence detection system, advanced flow cytometry software
This BD FACSymphony X-50 Flow Cytometer is a robust platform widely adopted in molecular biology and biotechnology laboratories, optimizing workflows for gene editing studies, cell phenotyping, and biopharmaceutical development.
Item Stats
Product details
| Stock | In Stock |
| Brand | BD |
| Product Code | 67238 |
| Condition | Pre-Owned |
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